Rapid and sensitive liquid chromatography with tandem mass spectrometry method for the simultaneous determination of 11 major components of Yuanhu‐Baizhi herb‐pair in rat perfusion fluids

• Published in: Journal of separation science Vol. 37; no. 12; pp. 1429 - 1437
• Main Authors: Liu, Yinli, Chen, Bin, Le, Jian, Xu, Yanhai, Song, Lin, Chai, Yifeng, Fan, Guorong, Hong, Zhanying
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH 01.06.2014; Blackwell Publishing Ltd; Wiley; Wiley Subscription Services, Inc
• Subjects: alkaloids; Animals; Biological and medical sciences; Chinese medicine; Chromatography; Chromatography, High Pressure Liquid - methods; coumarins; diazepam; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - analysis; Elution; Formates; Formic acid; General pharmacology; Herbal medicine; Injections, Intraperitoneal; intestinal absorption; Ionization; Linearity; Liquid chromatography; Liquid chromatography tandem mass spectrometry; Major components; Male; Mass spectrometry; Medical sciences; medicinal plants; monitoring; Perfusion; Perfusion fluids; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Rat perfusion fluids; Rats; Rats, Wistar; Sensitivity and Specificity; tandem mass spectrometry; Tandem Mass Spectrometry - methods; Yuanhu-Bazhi herb-pair; Asia; China; Tandem mass spectrometry; Yuanhu-Bazhi herb-pair; Chemical analysis; Rat; Coumarine derivatives; HPLC chromatography; Rat perfusion fluids; Electrospray; Medicinal plant; Alkaloid; Major components; Folk medicine; Dicotyledones; Angiospermae; Rhizome; Quantitative analysis; Below ground plant part; Validation; Liquid chromatography tandem mass spectrometry; Elution; Gradient; Angelica dahurica; Root; Coupled method; Pharmacognosy; Furocoumarine derivatives; Rodentia; Perfusion fluid; Fumariaceae; Vertebrata; Reversed phase chromatography; Mammalia; Animal; Simultaneous measurement; Plant origin; Active ingredient; Spermatophyta; Umbelliferae
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201400054

A rapid and sensitive liquid chromatography with tandem mass spectrometry method has been developed and validated for the simultaneous quantification of 11 major compounds in Yuanhu‐Bazhi herb‐pair from a rat intestinal perfusion experiment, including eight alkaloids and three coumarins using diazepam as the internal standard. The analysis was performed on an Agilent Poroshell 120EC‐C₁₈ column using a gradient elution of acetonitrile/H₂O with 0.0015% formic acid and 2 mM ammonium formate buffer additive (pH 4.2). All compounds were detected by tandem mass spectrometry in positive ionization mode and quantified using multiple reaction monitoring mode. Good linearity in the related range of each compound was presented. The validation data, such as accuracy and precision, were within the required limits. The lower limit of quantification of each analyte was less than 10 ng/mL. Intra‐day and inter‐day precisions were less than 12.84%. The relative errors of accuracy were in the range of –13.31 to 13.19%. The recoveries of analytes were greater than 84.08%. The method has been successfully applied to an intestinal absorption study of major components following intestinal perfusion of Yuanhu‐Bazhi herb‐pair extract to rats.