Characterization and sequence of a cDNA clone of gamma-glutamyltranspeptidase

• Published in: Nucleic acids research Vol. 14; no. 3; pp. 1393 - 1403
• Main Authors: Coloma, Julio, Pitot, Henry C.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 11.02.1986
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Biotechnology; cDNA; Chromosome Mapping; Cloning, Molecular; DNA - genetics; DNA Restriction Enzymes; Fundamental and applied biological sciences. Psychology; gamma -glutamyltransferase; gamma-Glutamyltransferase - genetics; genes; Genetic engineering; Genetic technics; Methods. Procedures. Technologies; nucleotide sequence; Rats; RNA, Messenger - genetics; Primary structure; Complementary DNA; Molecular cloning; γ-Glutamyltransferase
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/14.3.1393

We have isolated several cDNA's complementary to gamma-glutamyltranspeptidase (GOT) mRNA by screening a rat kidney library constructed in λgtll with antibodies specifically reactive to the enzyme protein. The clone selected an mRNA that was translated into a 62 Kd peptide, corresponding to the GGT precursor. The longest clone isolated was 1842 bp long with an open reading frame coding for 565 amino acids. The length of the mRNA coding for GGT was estimated to be 2.2 kb long. The amino acid sequence derived from the nucleotide sequence matched the short sequences determined by us as well as by other authors.