Kinetics of alpha-amylase synthesis from Bacillus amyloliquefaciens

The microbial production of alpha-amylase from Bacillus amyloliquefaciens was investigated. The microorganism was grown using media containing glucose or maltose at 37 degrees C and under aerobic conditions in a 16-L fermentor. The alpha-amylase synthesis from maltose was not found to be inducible b...

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Bibliographic Details
Published inBiotechnology and bioengineering Vol. 31; no. 4; p. 357
Main Authors Yoo, Y.J, Cadman, T.W, Hong, J, Hatch, R.T
Format Journal Article
LanguageEnglish
Published United States 01.03.1988
Subjects
AMILASAS
AMYLASE
AMYLASES
BACILLUS
MALTOSA
MALTOSE
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Summary:The microbial production of alpha-amylase from Bacillus amyloliquefaciens was investigated. The microorganism was grown using media containing glucose or maltose at 37 degrees C and under aerobic conditions in a 16-L fermentor. The alpha-amylase synthesis from maltose was not found to be inducible but was found to be subject to catabolite repression. The maltose uptake rate was observed to be the rate-limiting step compared to the conversion rate of maltose to glucose by intracellular alpha-glucosidase. The alpha-amylase activity achieved with maltose as a substrate was higher than that achieved with glucose. A slower growth rate and a higher cell density were obtained with maltose. The enzyme production pattern depended upon the nutrient composition of the medium.
Bibliography:8834961
Q02
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260310412