Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3'-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium

• Published in: Frontiers in microbiology Vol. 7; p. 1460
• Main Authors: Bondue, Pauline, Crèvecoeur, Sébastien, Brose, François, Daube, Georges, Seghaye, Marie-Christine, Griffiths, Mansel W, LaPointe, Gisèle, Delcenserie, Véronique
• Format: Journal Article Web Resource
• Language: English
• Published: Switzerland Frontiers Research Foundation 22.09.2016; Frontiers Media S.A
• Subjects: Bifidobacterium bifidum; Bifidobacterium crudilactis; bovine milk oligosaccharide; Escherichia coli enterohemorragic O157:H7; Life sciences; Microbiologie; Microbiology; Salmonella enterica serovar Typhimurium; Sciences du vivant; virulence expression; Bifidobacterium crudilactis; whey; Escherichia coli enterohemorragic O157:H7; bovine milk oligosaccharide; Salmonella enterica serovar Typhimurium; virulence expression; 3′-sialyllactose; Bifidobacterium bifidum
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2016.01460

Complex oligosaccharides from human milk (HMO) possess an antimicrobial activity and can promote the growth of bifidobacteria such as and subsp. . In addition, fermentation of carbohydrates by bifidobacteria can result in the production of metabolites presenting an antivirulence effect on several pathogenic bacteria. Whey is rich in complex bovine milk oligosaccharides (BMO) structurally similar to HMO and , a species of bovine origin, is able to metabolize some of those complex carbohydrates. This study focused on the ability of and to grow in a culture medium supplemented in 3'-sialyllactose (3'SL) as the main source of carbon, a major BMO encountered in cow milk. Next, the effects of cell-free spent media (CFSM) were tested against virulence expression of O157:H7 and serovar Typhimurium. Both strains were able to grow in presence of 3'SL, but showed the best growth (7.92 ± 0.3 log cfu/ml) compared to (6.84 ± 0.9 log cfu/ml). Then, CFSM were tested for their effects on virulence gene expression by and promoter activity of luminescent mutants of and . Typhimurium, respectively, and on wild type strains of O157:H7 and . Typhimurium using RT-qPCR. All CFSM resulted in significant under expression of the and genes for the luminescent mutants and (ratios of -15.4 and -8.1 respectively) and (ratios of -2.1 and -3.1) for the wild type strain of O157:H7. The 3'SL, a major BMO, combined with some bifidobacteria strains of bovine or human origin could therefore be an interesting synbiotic to maintain or restore the intestinal health of young children. These effects observed will be further investigated regarding the overall phenotype of pathogenic agents and the exact nature of the active molecules.