Expression levels of circulatory mir-185-5p, vascular endothelial growth factor, and platelet-derived growth factor target genes in endometriosis
Using blood-based biomarkers such as microRNAs (miRNAs) may allow particularly effective and minimally invasive diagnosis and treatment of endometriosis. We evaluated the differential expression of circulating miRNA-185-5p (miR-185-5p), vascular endothelial growth factor , and platelet-derived growt...
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Published in | International Journal of Reproductive BioMedicine Vol. 18; no. 5; pp. 347 - 358 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Iran
Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility
01.05.2020
Knowledge E Shahid Sadoughi University of Medical Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | Using blood-based biomarkers such as microRNAs (miRNAs) may allow particularly effective and minimally invasive diagnosis and treatment of endometriosis.
We evaluated the differential expression of circulating miRNA-185-5p (miR-185-5p), vascular endothelial growth factor
, and platelet-derived growth factor
target genes between endometriosis and healthy women.
25 women with a history of endometriosis (grad III-IV) diagnosed by laparoscopy as the case group and 25 women without endometriosis underwent laparoscopy for ovarian cysts or pelvic pain as the control group were enrolled in this case-control study. Blood samples were obtained, and total RNA was used for high-throughput small RNA sequencing, and this was confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR).
miRNA expression profiling using non-coding RNA sequencing revealed that one miRNA including miR-185-5p was significantly down-regulated in the case group compared with the controls. The qRT-PCR results showed significant downregulation of the expression level of miR-185-5p (
0.01) in the plasma of the case group. Receiver operating characteristic (ROC) curve analysis showed the area of miR-185-5p under the ROC curve for endometriosis diagnosis was 0.919 (
0.001). The RT-PCR results demonstrated that there was no significant difference in the expression of
and
mRNA of blood samples in the cases compared to the control group (
,
= 0.09 and
,
= 0.36).
The low expression of miR-185-5p in the plasma of women with endometriosis could be employed as an important non-invasive biomarker for early detection and screening of endometriosis by blood samples. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2476-4108 2476-3772 |
DOI: | 10.18502/ijrm.v13i5.7155 |