Relationship between (Na + K)-ATPase activity, lipid peroxidation and fatty acid profile in erythrocytes of hypertensive and normotensive subjects

• Published in: Molecular and cellular biochemistry Vol. 303; no. 1-2; pp. 73 - 81
• Main Authors: Rodrigo, Ramón, Bächler, Jean P, Araya, Julia, Prat, Hernán, Passalacqua, Walter
• Format: Journal Article
• Language: English
• Published: Netherlands Boston : Springer US 01.09.2007; Springer Nature B.V
• Subjects: (Na + K)-ATPase; Adult; Blood pressure; Blood Pressure Monitoring, Ambulatory; Case-Control Studies; Cross-Sectional Studies; Erythrocyte Membrane - chemistry; Erythrocytes; Erythrocytes - metabolism; Essential hypertension; Fatty acids; Fatty Acids - metabolism; Glutathione - metabolism; Humans; Hypertension; Hypertension - metabolism; Hypertension - pathology; Lipid Peroxidation; Male; Malondialdehyde - metabolism; Membranes; Middle Aged; Oxidative Stress; Peroxidation; Plasma; Sodium-Potassium-Exchanging ATPase - metabolism
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-007-9457-y

Oxidative stress may play a role in the pathogenic mechanism of essential hypertension. Lipid peroxidation can alter the cellular structure of membrane-bound enzymes by changing the membrane phospholipids fatty acids composition. We investigated the relationship between (Na + K)-ATPase activity, lipid peroxidation, and erythrocyte fatty acid composition in essential hypertension. The study included 40 essential hypertensive and 49 healthy normotensive men (ages 35-60 years). Exclusion criteria were obesity, dyslipidemia, diabetes mellitus, smoking, and any current medication. Patients underwent 24-h ambulatory blood pressure monitoring and blood sampling. Lipid peroxidation was measured in the plasma and erythrocytes as 8-isoprostane or malondialdehyde (MDA), respectively. Antioxidant capacity was measured as ferric reducing ability of plasma (FRAP) in the plasma and as reduced/oxidized glutathione (GSH/GSSG ratio) in erythrocytes. (Na + K)-ATPase activity and fatty acids were determined in erythrocyte membranes. Hypertensives had higher levels of plasma 8-isoprostane, erythrocyte MDA, and relative percentage of saturated membrane fatty acids, but lower plasma FRAP levels, erythrocyte GSH/GSSG ratio, (Na + K)-ATPase activity and relative percentage of unsaturated membrane fatty acids, compared with normotensives. Day-time systolic and diastolic blood pressures correlated positively with lipid peroxidation parameters, but negatively with (Na + K)-ATPase activity. These findings suggest that the modulation of (Na + K)-ATPase activity may be associated with changes in the fatty acid composition induced by oxidative stress and provide evidence of a role for this enzyme in the pathophysiology of essential hypertension.