Characterization and expression of caffeoyl-coenzyme A 3-O-methyltransferase proposed for the induced resistance response of Vitis vinifera L

• Published in: Plant physiology (Bethesda) Vol. 115; no. 3; pp. 1039 - 1048
• Main Authors: Busam, G, Junghanns, K.T, Kneusel, R.E, Kassemeyer, H.H, Matern, U
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.11.1997
• Subjects: 2,6-DICHLOROISONICOTINIC ACID; ACIDE CAFEIQUE; ACIDE PHENOLIQUE; ACIDE SALICYLIQUE; ACIDO CAFEICO; ACIDO SALICILICO; ACIDOS FENOLICOS; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ADN; AGENT PATHOGENE; Amino Acid Sequence; AMINO ACID SEQUENCES; Base Sequence; BENZO (1,2,3)-THIADIAZOLE-7-CARBOTHIOIC ACID S-METHYL ESTER; Biological and medical sciences; CAFFEIC ACID; CELL CULTURE; Cell culture techniques; CELL SUSPENSIONS; Cell walls; Cells, Cultured; CHEMICAL COMPOSITION; CHITINASE; CLONE; CLONES; Cloning, Molecular; COENZIMAS; COENZYME; COENZYMES; COMPLEMENTARY DNA; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Cucumbers; CULTIVO DE CELULAS; CULTURE DE CELLULE; Cultured cells; DERIVATIVES; DISEASE MARKERS; DISEASE RESISTANCE; DNA; DNA, Complementary; ENFERMEDADES FUNGOSAS; Enzymes; ENZYMIC ACTIVITY; Escherichia coli - genetics; ESTER; ESTERES; ESTERS; EXPRESION GENICA; EXPRESSION DES GENES; FENOTIPOS; Fundamental and applied biological sciences. Psychology; FUNGAL DISEASES; Fungal plant pathogens; GENBANK/Z54233; GENE; GENE EXPRESSION; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Gene Regulation and Molecular Genetics; GENES; GENETIC REGULATION; GENETICA; GENETICS; GENETIQUE; INDUCTION; LIGNIFICACION; LIGNIFICATION; MALADIE FONGIQUE; METHYLATION; Methyltransferases - genetics; Methyltransferases - metabolism; METILACION; MOLECULAR SEQUENCE DATA; NICOTINAMIDA; NICOTINAMIDE; NICOTINIC ACID; ORGANISMOS PATOGENOS; Parsley; PATHOGENESE; PATHOGENESIS; PATHOGENICITY; PATHOGENS; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; PATOGENESIS; PATOGENICIDAD; PHENOLIC ACIDS; PHENOTYPE; PHENOTYPES; Phytopathology. Animal pests. Plant and forest protection; Plant cells; Plants; POUVOIR PATHOGENE; PROTEINAS; PROTEINE; PROTEINS; QUITINASA; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; RNA; Rosales - enzymology; Rosales - genetics; SALICYLIC ACIDS; Sequence Homology, Amino Acid; SINTOMAS; SYMPTOME; SYMPTOMS; SYSTEMIC ACQUIRED RESISTANCE; TRANSFERASAS; TRANSFERASE; TRANSFERASES; VITIS VINIFERA; Cell culture; Plant pathogen; Homology; Induced resistance; Vitis vinifera; Caffeoyl-CoA O-methyltransferase; Fungi; Microorganism resistance; Lignification; Enzymatic activity; Vitidaceae; Dicotyledones; Substrate specificity; Angiospermae; Elicitation; Aminoacid sequence; Thallophyta; Heterologous system; Nucleotide sequence; Enzyme; Transferases; Gene expression; Methyltransferases; Complementary DNA; Fruit crop; Phytoalexin; Spermatophyta; Biological accumulation
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.115.3.1039

Cell-suspension cultures of Vitis vinifera L. cv Pinot Noir accumulated resveratrol upon fungal elicitation, and the activity of S-adenosyl-1-methionine:trans-caffeoyl-coenzyme A 3-O-methyltransferase (CCoAOMT), yielding feruloyl-CoA, increased to a transient maximum at 12 to 15 h. CCoAOMT cDNA was cloned from the elicited cells and was shown to encode a polypeptide highly homologous to CCoAOMTs from cells of Petroselinum species or Zinnia species. The expression of the cDNA in Escherichia coli revealed that grapevine CCoAOMT methylates both caffeoyl- and 5-hydroxyferuloyl-coenzyme A and is probably involved in phenolic esterification and lignification. Commercial plant activators induce the disease-resistance response of test plants and are considered to mimic the action of salicylic acid. Among these chemicals, 2,6-dichloroisonicotinic acid and benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester provoke systemic acquired resistance (SAR) and were also shown to induce the expression of class III chitinase in grapevine. The SAR response is classified by an unchanged phenotype of tissues, but the mechanistic basis is unknown. Treatment of the cultured V. vinifera cells with either fungal elicitor or low concentrations of salicylic acid and 2,6-dichloroisonicotinic acid, respectively, raised the CCoAOMT or stilbene synthase transcript abundance, suggesting that grapevine is capable of the SAR response, whereas benzo(1,2,3)-thiadiazole7-carbothioic acid S-methyl ester was ineffective. The data imply for the first time (to our knowledge) that the expression of phenylpropanoid genes in grapevine is induced by SAR activators without phenotypic consequences and suggest a role for CCoAOMT and stilbene synthase in the disease-resistance response leading beyond the level of pathogenesis-related proteins as markers of the SAR