Monitoring Wind-Borne Particle Matter Entering Poultry Farms via the Air-Inlet: Highly Pathogenic Avian Influenza Virus and Other Pathogens Risk

• Published in: Pathogens (Basel) Vol. 11; no. 12; p. 1534
• Main Authors: Elbers, Armin R W, Gonzales, José L, Koene, Miriam G J, Germeraad, Evelien A, Hakze-van der Honing, Renate W, van der Most, Marleen, Rodenboog, Henk, Velkers, Francisca C
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14.12.2022; MDPI
• Subjects: Avian flu; Avian influenza viruses; bird feathers; Birds; Campylobacter; cobweb; Control; Culicidae; Diseases; Epidemics; faecal material; Farms; Genetic aspects; Growth; Health aspects; Health risks; Houses; Identification and classification; Infections; Influenza; Inlets; Insects; Intensive farming; Monitoring; Mosquitoes; particle matter; Particles; Pathogenic microorganisms; Pathogens; plant; Poultry; Poultry farming; Poultry housing; Residential areas; Salmonella; Ventilation; Viruses; Wind; Netherlands; North Sea; Salmonella; highly pathogenic avian influenza; cobweb; particle matter; bird feathers; avian influenza virus; plant; wild aquatic avian species; insects; faecal material; Campylobacter
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens11121534

Wind-supported transport of particle matter (PM) contaminated with excreta from highly pathogenic avian influenza virus (HPAIv)-infected wild birds may be a HPAIv-introduction pathway, which may explain infections in indoor-housed poultry. The primary objective of our study was therefore to measure the nature and quantity of PM entering poultry houses via air-inlets. The air-inlets of two recently HPAIv-infected poultry farms (a broiler farm and a layer farm) were equipped with mosquito-net collection bags. PM was harvested every 5 days for 25 days. Video-camera monitoring registered wild bird visits. PM was tested for avian influenza viruses (AIV), and with PCR. Insects, predominantly mosquitoes, were tested for AIV, West Nile, Usutu and Schmallenberg virus. A considerable number of mosquitoes and small PM amounts entered the air-inlets, mostly cobweb and plant material, but no wild bird feathers. Substantial variation in PM entering between air-inlets existed. In stormy periods, significantly larger PM amounts may enter wind-directed air-inlets. PM samples were AIV and negative and insect samples were negative for all viruses and bacteria, but several broiler and layer farm PM samples tested positive. Regular wild (water) bird visits were observed near to the poultry houses. Air-borne PM and insects-potentially contaminated with HPAIv or other pathogens-can enter poultry air-inlets. Implementation of measures limiting this potential introduction route are recommended.