Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 19; p. 6514
• Main Authors: Takács, Eszter, Gémes, Borbála, Szendrei, Fanni, Keszei, Csaba, Barócsi, Attila, Lenk, Sándor, Domján, László, Mörtl, Mária, Székács, András
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.10.2022; MDPI
• Subjects: Agricultural pollution; Antibodies; Aquatic ecosystems; Calibration; Chemical properties; Chromatography; competitive immunoassay; Cross-reaction; Dilution; Ecosystem; ELFIA; Environmental aspects; Environmental Pollutants; Enzyme-linked immunosorbent assay; Enzymes; Fluorescence; fluorescence detection; Fluorescent Antibody Technique; Glycine - analogs & derivatives; Glyphosate; Herbicides; Herbicides - analysis; Immunoassay; Immunofluorescence; Instrumentation; Laboratories; Metabolites; Methods; Pesticides; Plant tissues; Pollutants; Project Aquafluosense; Signal detection; Soil; Soil pollution; Soil water; Surface water; Terrestrial ecosystems; Visual signals; Water analysis; Water quality; Water sampling; Xenobiotics; Hungary; Project Aquafluosense; ELFIA; competitive immunoassay; fluorescence detection; glyphosate
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27196514

An enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the quantitative analytical determination of the herbicide active ingredient glyphosate in environmental matrices (surface water, soil, and plant tissues). Glyphosate, as a ubiquitous agricultural pollutant, is a xenobiotic substance with exposure in aquatic and terrestrial ecosystems due its extremely high worldwide application rate. The immunoassay developed in Project Aquafluosense is part of a fluorescence-based instrumentation setup for the in situ determination of several characteristic water quality parameters. The 96-well microplate-based competitive immunoassay method applies fluorescence signal detection in the concentration range of 0-100 ng/mL glyphosate. Application of the fluorescent signal provides a limit of detection of 0.09 ng/mL, which is 2.5-fold lower than that obtained with a visual absorbance signal. Beside the improved limit of detection, determination by fluorescence provided a wider and steeper dynamic range for glyphosate detection. No matrix effect appeared for the undiluted surface water samples, while plant tissues and soil samples required dilution rates of 1:10 and 1:100, respectively. No cross-reaction was determined with the main metabolite of glyphosate, N-aminomethylphosphonic acid, and related compounds.