Differential Expression of Ly6C and T-bet Distinguish Effector and Memory Th1 CD4 + Cell Properties during Viral Infection

• Published in: Immunity (Cambridge, Mass.) Vol. 35; no. 4; pp. 633 - 646
• Main Authors: Marshall, Heather D., Chandele, Anmol, Jung, Yong Woo, Meng, Hailong, Poholek, Amanda C., Parish, Ian A., Rutishauser, Rachel, Cui, Weiguo, Kleinstein, Steven H., Craft, Joe, Kaech, Susan M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 28.10.2011; Elsevier Limited
• Subjects: Animals; Antigens, Ly - genetics; Antigens, Ly - immunology; Cell Proliferation; Gene expression; Gene Expression Regulation; Homeostasis; Immunologic Memory; Infections; Lymphocytic choriomeningitis virus; Mice; Mice, Inbred C57BL; T cell receptors; T-Box Domain Proteins - genetics; T-Box Domain Proteins - immunology; Th1 Cells - cytology; Th1 Cells - immunology; Th1 Cells - virology; Viral infections
• ISSN: 1074-7613; 1097-4180
• DOI: 10.1016/j.immuni.2011.08.016

CD4 + T cells differentiate into multiple effector types, but it is unclear how they form memory T cells during infection in vivo. Profiling virus-specific CD4 + T cells revealed that effector cells with T helper 1 (Th1) or T follicular helper (Tfh) cell characteristics differentiated into memory cells, although expression of Tfh cell markers declined over time. In contrast to virus-specific effector CD8 + T cells, increased IL-7R expression was not a reliable marker of CD4 + memory precursor cells. However, decreased Ly6C and T-bet ( Tbx21) expression distinguished a subset of Th1 cells that displayed greater longevity and proliferative responses to secondary infection. Moreover, the gene expression profile of Ly6C loT-bet int Th1 effector cells was virtually identical to mature memory CD4 + T cells, indicating early maturation of memory CD4 + T cell features in this subset during acute viral infection. This study provides a framework for memory CD4 + T cell development after acute viral infection. [Display omitted] ► Increased IL-7R expression does not mark CD4 + memory precursor cells ► T-bet acts in a graded manner to regulate formation of Th1 and Tfh cell subsets ► Ly6C loT-bet int Th1 effector CD4 + cells have enhanced longevity and recall responses ► Ly6C loT-bet int Th1 effector and memory CD4 + cells share similar gene expression