Comparative neuronal differentiation of self-renewing neural progenitor cell lines obtained from human induced pluripotent stem cells

Most human neuronal disorders are associated with genetic alterations that cause defects in neuronal development and induce precocious neurodegeneration. In order to fully characterize the molecular mechanisms underlying the onset of these devastating diseases, it is important to establish in vitro...

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Published inFrontiers in cellular neuroscience Vol. 7; p. 175
Main Authors Verpelli, Chiara, Carlessi, Luigi, Bechi, Giulia, Fusar Poli, Elena, Orellana, Daniel, Heise, Christopher, Franceschetti, Silvana, Mantegazza, Renato, Mantegazza, Massimo, Delia, Domenico, Sala, Carlo
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Research Foundation 2013
Frontiers Media S.A
Subjects
Cell culture
Cell differentiation
Cell lines
Cloning
Fibroblasts
Glial cells
Human neurons
Immunofluorescence
Induced Pluripotent Stem Cells
Molecular modelling
Neural stem cells
Neurodegeneration
neuronal differentiation
Neuronal-glial interactions
Neuroscience
Parkinson's disease
Penicillin
Pluripotency
postsynapse
Progenitor cells
Stem cell transplantation
Stem cells
synapse formation
Italy
induced pluripotent stem cells
Human neurons
postsynapse
neuronal differentiation
synapse formation
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Summary:Most human neuronal disorders are associated with genetic alterations that cause defects in neuronal development and induce precocious neurodegeneration. In order to fully characterize the molecular mechanisms underlying the onset of these devastating diseases, it is important to establish in vitro models able to recapitulate the human pathology as closely as possible. Here we compared three different differentiation protocols for obtaining functional neurons from human induced pluripotent stem cells (hiPSCs): human neural progenitors (hNPs) obtained from hiPSCs were differentiated by co-culturing them with rat primary neurons, glial cells or simply by culturing them on matrigel in neuronal differentiation medium, and the differentiation level was compared using immunofluorescence, biochemical and electrophysiological methods. We show that the differentiated neurons displayed distinct maturation properties depending on the protocol used and the faster morphological and functional maturation was obtained when hNPs were co-cultured with rat primary neurons.
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Reviewed by: Dorit Cohen-Carmon, Hebrew University of Jerusalem, Israel
This article was submitted to the journal Frontiers in Cellular Neuroscience.
Edited by: Eran Meshorer, The Hebrew University of Jerusalem, Israel
ISSN:1662-5102
1662-5102
DOI:10.3389/fncel.2013.00175