Characterization of a variant CD4 molecule in Japanese Black cattle

• Published in: Veterinary Immunology and Immunopathology Vol. 232; p. 110167
• Main Authors: Kato-Mori, Yuko, Okamura, Taku, Kawashita, Norihito, Hagiwara, Katsuro
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2021; Elsevier BV
• Subjects: Amino Acid Substitution; amino acids; Animals; Antibodies, Monoclonal; Blood Cell Count; Cattle; CD4; CD4 Antigens; cytokines; epitopes; farms; Flow Cytometry; gene expression; genes; glycoproteins; immunopathology; Japanese Black cattle; Leukocytes, Mononuclear; macrophages; mitogens; Models, Molecular; Molecular Structure; monocytes; Mutation; Variant; Wagyu; TCR; MHC; DP; IFN; FITC; Th; OD; SI; DDBJ; PBS; CD; BSS; IL; WC1; Japanese Black cattle; PBMC; mAb; Variant; FCM; CD4; FCS; JB; RPMI; GAPDH; Ig; PCR; ConA

•PBMCs from Japanese Black calves were analyzed using two anti-CD4 mAbs.•A CD4 variant was found to contain 4 point mutations.•The 4 point mutations resulted in 3 amino acid substitutions.•The mutations did not affect lymphocyte functions. Monoclonal antibodies (mAbs) that recognize cluster of diffe...