Agaricus blazei extract (FA‑2‑b‑β) induces apoptosis in chronic myeloid leukemia cells

Agaricus blazei Murill (AbM) is a mushroom belonging to the Basidiomycetes family, which is believed to have antitumor and antioxidative activities. Proteoglycans and ergosterol are considered the key compounds of AbM for antitumor properties and so are used in complementary and alternative medicine...

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Published inOncology letters Vol. 20; no. 5; p. 1
Main Authors Sun, Yanqing, Cheng, Mingxia, Dong, Li, Yang, Kehu, Ma, Zhiyuan, Yu, Shangrui, Yan, Peijing, Bai, Kuntian, Zhu, Xiaolong, Zhang, Qike
Format Journal Article
LanguageEnglish
Published Athens Spandidos Publications 01.11.2020
Spandidos Publications UK Ltd
D.A. Spandidos
Subjects
Alternative medicine
Antibodies
Apoptosis
Biotechnology industries
Cancer
Cell cycle
Cell growth
Chemotherapy
Chronic myeloid leukemia
Flow cytometry
Kinases
Leukemia
Oncology
Phytosterols
Stem cells
Japan
China
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Summary:Agaricus blazei Murill (AbM) is a mushroom belonging to the Basidiomycetes family, which is believed to have antitumor and antioxidative activities. Proteoglycans and ergosterol are considered the key compounds of AbM for antitumor properties and so are used in complementary and alternative medicine as an anticancer drug. AbM is used to avoid serious side effects that would inevitably affect patients. Currently, the efficacy of AbM against chronic myeloid leukemia (CML) has not been established. The present study aimed to investigate the antitumor activities of the acidic RNA protein complex, FA-2-b-[beta], extracted from wild edible AbM. The CML K562 cells or primary CML bone marrow (BM) cells were treated with FA-2-b-[beta] at different concentrations and time points. CML cell line proliferation and apoptosis were determined using the CCK-8 assay or Annexin V/propidium iodide (PI) labeling, RT-qPCR and western blotting was performed to determine the involvement of the Wnt/[beta]-catenin-associated apoptotic pathway. The results of the present study demonstrated that FA-2-b-[beta] has a high anti-proliferative potency and strong pro-apoptotic effects. Thus, daily intake of mushrooms containing FA-2-b-[beta] may be an adequate source as an alternative medicine in the management of CML, and may provide useful information for the development of a novel therapeutic target in this area. Key words: CML, AbM, Wnt/[beta]-catenin signaling pathway, apoptosis cycle
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ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2020.12133