Microbacterium xylanilyticum sp. nov., a xylan-degrading bacterium isolated from a biofilm

• Published in: International journal of systematic and evolutionary microbiology Vol. 55; no. 5; pp. 2075 - 2079
• Main Authors: Kim, Kwang Kyu, Park, Hye Yoon, Park, Wooshin, Kim, In S, Lee, Sung-Taik
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.09.2005; Society for General Microbiology
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Actinomycetales - physiology; Bacterial Typing Techniques; Bacteriology; Biodegradation, Environmental; Biofilms - growth & development; Biological and medical sciences; Bioreactors; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Microbacterium foliorum; Microbacterium xylanilyticum; Microbiology; Miscellaneous; Molecular Sequence Data; Nucleic Acid Hybridization; Phenotype; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Systematics; Waste Disposal, Fluid - methods; Xylans - metabolism; Biofilm; Xylan; Bacteria; Actinomycetes; Microbacterium; Taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63706-0

1 Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea 2 Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 1 Oryong-dong, Buk-gu, Gwangju 500-712, Republic of Korea Correspondence Sung-Taik Lee e_stlee{at}kaist.ac.kr A novel xylan-degrading bacterium, S3-E T , was isolated from the biofilm of a membrane bioreactor. The cells of this strain were Gram-positive, non-motile, non-spore-forming rods, produced primary branches and formed yellow colonies on nutrient agar. The strain had chemotaxonomic markers that were consistent with classification in the genus Microbacterium , i.e. MK-12, MK-11 and MK-13 as the major menaquinones, predominant iso- and anteiso-branched cellular fatty acids, glucose and galactose as the cell-wall sugars, peptidoglycan-type B2 with glycolyl residues and a DNA G+C content of 69·7 mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain S3-E T is most similar to Microbacterium hominis IFO 15708 T and Microbacterium foliorum DSM 12966 T (97·6 and 97·4 % sequence similarity, respectively), and that it forms a separate lineage with M. hominis in the genus Microbacterium . DNA–DNA hybridization results and phenotypic properties showed that strain S3-E T could be distinguished from all known Microbacterium species and represented a novel species, for which the name Microbacterium xylanilyticum sp. nov. is proposed; the type strain is S3-E T (=DSM 16914 T =KCTC 19079 T ). Published online ahead of print on 27 May 2005 as DOI 10.1099/ijs.0.63706-0. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Microbacterium xylanilyticum S3-E T is AJ853908 . A phase-contrast micrograph of S3-E T cells and an extended version of the 16S rRNA gene phylogenetic tree are available as supplementary figures in IJSEM Online.