A New PNA-FISH Probe Targeting Fannyhessea vaginae
Bacterial vaginosis (BV) is the most common vaginal infection in women of reproductive age and has been associated with serious health complications, mainly in pregnant women. It is characterized by a decrease in the number of species in the healthy vaginal microbiota and an overgrowth of strict and...
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Published in | Frontiers in cellular and infection microbiology Vol. 11; p. 779376 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
18.11.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Bacterial vaginosis (BV) is the most common vaginal infection in women of reproductive age and has been associated with serious health complications, mainly in pregnant women. It is characterized by a decrease in the number of
species in the healthy vaginal microbiota and an overgrowth of strict and facultative anaerobic bacteria that develop a polymicrobial biofilm. Despite over 60 years of research investigating BV, its etiology is not fully understood.
spp. is a crucial microorganism that contributes to the formation of the biofilm and the development of BV, but the role of other BV-associated bacteria is not clear. Nevertheless,
(previously known as
) is a highly specific species for BV, and co-colonization with
is thought to be a very specific diagnostic marker. The diagnosis of BV still presents some limitations, since currently used methods often fail to accurately detect BV. This work aims to develop a novel peptide nucleic acid (PNA) probe targeting
. This probe was further validated in a multiplex assay, which included a
specific PNA probe, as a possible method for diagnosis of BV, and was compared with quantification by qPCR. The new PNA probe showed excellent sensitivity and specificity and could discriminate
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biofilms, confirming the potential to be used for the detection of BV-associated pathogens. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: António Machado, Universidad San Francisco de Quito, Ecuador Reviewed by: Milda Pleckaityte, Vilnius University, Lithuania; David Esteban Pacha-Herrera, University of Debrecen, Hungary This article was submitted to Biofilms, a section of the journal Frontiers in Cellular and Infection Microbiology |
ISSN: | 2235-2988 2235-2988 |
DOI: | 10.3389/fcimb.2021.779376 |