DNA ligases from thermophilic bacteria enhance PCR amplification of long DNA sequences

Bacterial NAD-dependent Taq and Tth DNA ligases are capable of significantly increasing the yield of long PCR products when the amplification is carried out using bacterial family A DNA polymerases, e.g. Taq or Tth DNA polymerases, or with enzymatic blends containing these polymerases. We also show...

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Published inBiochemistry (Moscow) Vol. 74; no. 5; pp. 557 - 561
Main Authors Ignatov, K. B, Kramarov, V. M
Format Journal Article
LanguageEnglish
Published Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01.05.2009
SP MAIK Nauka/Interperiodica
Springer Nature B.V
Subjects
Bacteria
Bacterial Proteins - metabolism
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Deoxyribonucleic acid
DNA
DNA - genetics
DNA Ligases - metabolism
DNA-Directed DNA Polymerase - metabolism
Enzymes
Life Sciences
Microbiology
NAD - metabolism
Polymerase Chain Reaction
Polymerization
Proteins
Taq Polymerase - metabolism
Thermus - enzymology
Taq DNA ligase
long PCR
Taq DNA polymerase
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Summary:Bacterial NAD-dependent Taq and Tth DNA ligases are capable of significantly increasing the yield of long PCR products when the amplification is carried out using bacterial family A DNA polymerases, e.g. Taq or Tth DNA polymerases, or with enzymatic blends containing these polymerases. We also show that Taq and Tth DNA ligases improve the results of PCR in the absence of NAD and therefore in the absence of DNA ligase activity. These observations suggest that bacterial DNA ligases can interact with these DNA polymerases, presumably as accessory proteins, thereby enhancing the efficiency of DNA polymerization.
Bibliography:http://dx.doi.org/10.1134/S0006297909050113
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297909050113