Development of an Ex Vivo Assay for Identification of Infectious Hepatitis E Virus in Different Kinds of Food Samples

Hepatitis E virus (HEV) is a positive-sense single-stranded RNA virus and a major cause of acute viral hepatitis. HEV is responsible for 20 million infections worldwide in humans every year. HEV-3 and HEV-4 are zoonotic and are responsible for most of the HEV cases in developed countries. Consumptio...

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Published inPathogens (Basel) Vol. 12; no. 10; p. 1231
Main Authors Hakze-van der Honing, Renate W, van Oort, Sophie, Dirks, René A. M, van der Poel, Wim H. M
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.10.2023
MDPI
Subjects
Animal welfare
Biochemical assays
Cell culture
Control
Developed countries
Experiments
Food consumption
Food contamination
Food contamination & poisoning
Foodborne diseases
Health risks
Hepatitis
Hepatitis E
Hepatitis E virus
Hepatocytes
HEV
Hogs
Infections
infectivity
Liver
primary hepatocytes
Risk assessment
Risk factors
RNA viruses
Strains (organisms)
Testing
Viruses
Netherlands
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Summary:Hepatitis E virus (HEV) is a positive-sense single-stranded RNA virus and a major cause of acute viral hepatitis. HEV is responsible for 20 million infections worldwide in humans every year. HEV-3 and HEV-4 are zoonotic and are responsible for most of the HEV cases in developed countries. Consumption of contaminated pig meat or pig products is considered to be the main transmission route of HEV HEV-3 in Europe. Prevalence studies for HEV generally use PCR methods to detect the presence or absence of genomic RNA. However, these methods do not discriminate infectious virus particles from non-infectious material. Previously developed HEV cell culture systems only worked with high efficiency after cell line adaptation of the subjected virus strains. In this manuscript, the development of a culture system for the detection of infectious HEV strains is described. For this purpose, we optimized the isolation and the growth of primary hepatocytes from young piglets. Subsequently, the isolated hepatocytes were used to culture HEV of different origins, such as liver tissue samples and sausage samples. This method can be applied to better assess the risk of infection through consumption of food products associated with HEV RNA contamination.
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ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens12101231