Urinary 8-oxo-7,8-dihydroguanosine as a Potential Biomarker of Aging

• Published in: Frontiers in aging neuroscience Vol. 10; p. 34
• Main Authors: Gan, Wei, Liu, Xin-Le, Yu, Ting, Zou, Yuan-Gao, Li, Ting-Ting, Wang, Shuang, Deng, Jin, Wang, Lan-Lan, Cai, Jian-Ping
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Research Foundation 27.02.2018; Frontiers Media S.A
• Subjects: 8-oxoguanosine; Age; Aging; Biomarkers; biomarkers of aging; Chromatography; Deoxyguanosine; Deoxyribonucleic acid; DNA; DNA methylation; Gene expression; Guanosine; High-performance liquid chromatography; Hospitals; Laboratories; Mass spectrometry; Metabolism; Metabolites; method validation; Neuroscience; Oxidative stress; Proteins; Scientific imaging; Senescence; Sex differences; Urine; Beijing China; United States > US; China; method validation; mass spectrometry; 8-oxoguanosine; biomarkers of aging; urine
• ISSN: 1663-4365; 1663-4365
• DOI: 10.3389/fnagi.2018.00034

A molecular biomarker of physiologic age, as opposed to chronologic age, is needed in clinical medicine. 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGsn) and 8-oxo-7, 8-dihydroguanosine (8-oxoGsn) are two promising aging biomarkers. A total of 1,228 healthy Chinese residents (613 males and 615 females) 2-90 years of age were randomly selected. Spot urine samples were collected, and the concentrations of 8-oxodGsn and 8-oxoGsn were measured using ultra-high-performance liquid chromatography with a triple quadrupole mass spectrometer (UPLC-MS/MS). Method validation, including accuracy, precision, linearity and quantification limit, was performed. The relationship between oxidized guanosine and age/gender was evaluated. 8-oxodGsn and 8-oxoGsn were eluted at 1.61 and 1.30 min, respectively. The calibration curve was linear in the range of 0.2-500 ng/ml for both analytes. The lowest limit of quantification (LLOQ) was 0.2 ng/ml for 8-oxodGsn and 0.1 ng/ml for 8-oxoGsn. There was an age-dependent increase in the biomarkers from the 21- to 30-year-old group to the 81- to 90-year-old group in both genders. In the subjects older than 61 years of age, the levels of 8-oxodGsn as well as 8-oxoGsn in urine were much higher in females than in males. The content of 8-oxoGsn correlated more closely with age and was higher (approximately 2-fold) than that of 8-oxodGsn for a given individual. 8-oxodGsn and 8-oxoGsn can be easily measured by UPLC-MS/MS. Urinary 8-oxoGsn may be a potential biomarker to determine a person's physiologic age and identify individuals at high risk of developing age-associated disease.