Cytokine adsorption/release on uniform magnetic nanoparticles for localized drug delivery

• Published in: Journal of controlled release Vol. 130; no. 2; pp. 168 - 174
• Main Authors: Mejías, Raquel, Costo, Rocío, Roca, Alejandro G., Arias, Cristina F., Veintemillas-Verdaguer, Sabino, González-Carreño, Teresita, del Puerto Morales, María, Serna, Carlos J., Mañes, Santos, Barber, Domingo F.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10.09.2008; Elsevier
• Subjects: Adsorption; Animals; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Biological and medical sciences; Cytokines; Drug Carriers - chemistry; Drug Compounding; Drug delivery; Drug Delivery Systems - methods; Drug Stability; General pharmacology; Hydrogen-Ion Concentration; Immunomodulation; Interferon-gamma - chemistry; Interferon-gamma - pharmacology; Interferon-gamma - therapeutic use; Iron oxide; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - immunology; Magnetics; Medical sciences; Mice; Mice, Inbred C57BL; Microscopy, Electron, Transmission; Nanoparticle; Nanoparticles - chemistry; Neoplasms - drug therapy; Neoplasms - immunology; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Surface modifications; Surface Properties; X-Ray Diffraction; Surface modifications; Drug delivery; Iron oxide; Cytokines; Nanoparticle; Immunomodulation; Drug; Pharmaceutical technology; Cytokine; Drug carrier; Magnetic nanomaterial; Local administration; Adsorption; Microparticle; Release; Gamma interferon
• ISSN: 0168-3659; 1873-4995
• DOI: 10.1016/j.jconrel.2008.05.028

Attachment of cytokines to magnetic nanoparticles has been developed as a system for controlled local drug release in cancer therapy. We studied the adsorption/release of murine interferon gamma (IFN-γ) on negatively charged magnetic nanoparticles prepared by three different methods, including coprecipitation, decomposition in organic media, and laser pyrolysis. To facilitate IFN-γ adsorption, magnetic nanoparticles were surface modified by distinct molecules to achieve high negative charge at pH 7, maintaining small aggregate size and stability in biological media. We analyzed carboxylate-based coatings and studied the colloidal properties of the resulting dispersions. Finally, we incubated the magnetic dispersions with IFN-γ and determined optimal conditions for protein adsorption onto the particles, as well as the release capacity at different pH and as a function of time. Particles prepared by decomposition in organic media and further modified with dimercaptosuccinic acid showed the most efficient adsorption/release capacity. IFN-γ adsorbed on these nanoparticles would allow concentration of this protein or other biomolecules at specific sites for treatment of cancer or other diseases.