Human nasal epithelial cells derived from multiple subjects exhibit differential responses to H3N2 influenza virus infection in vitro

• Published in: Journal of allergy and clinical immunology Vol. 138; no. 1; pp. 276 - 281.e15
• Main Authors: Yan, Yan, Tan, Kai Sen, Li, Chunwei, Tran, Thai, Chao, Siew Shuen, Sugrue, Richard J., Shi, Li, Chow, Vincent T., Wang, De-Yun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2016; Elsevier Limited
• Subjects: Allergy and Immunology; Cells, Cultured; Epithelial Cells - pathology; Epithelial Cells - virology; Gene expression; Humans; Infections; Influenza; Influenza A Virus, H3N2 Subtype - growth & development; Influenza, Human - metabolism; Influenza, Human - pathology; Nasal Mucosa - pathology; Nasal Mucosa - virology; Proteins; Review boards; Software; Studies; Viral infections; Viruses
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2015.11.016

[...]to elucidate the sequential and spatiotemporal reactions of pathogen sensors to respiratory viruses documented in different experimental models,9,E5,E13-E20 we performed analyses of (1) IAV receptors possessing terminal α-2,6-linked or α-2,3-linked sialic acid residues (α-2,6-SA or α-2,3-SA) and the rhinovirus receptor intercellular adhesion molecule 1; (2) the extracellular receptor Toll-like receptor (TLR) 4, the orphan human receptor TLR10, and the cytosolic receptors TLR3, TLR7, TLR8, and TLR9 located in the endosome; (3) the cytoplasmic sensors retinoic acid-inducible gene 1 (RIG-I) and NOD-like receptor family, pyrin domain containing 3; and (4) cytoplasmic molecules for sensing, including interferon regulatory factor (IRF) 3 and IRF7 and IFN-β promoter stimulator 1.