Linkage Mapping of a Mouse Gene, iv, that Controls Left-Right Asymmetry of the Heart and Viscera

Inherited single gene defects have been identified in both humans and mice that lead to loss of developmental control over the left-right asymmetry of the heart and viscera. In mice the recessively inherited mutation iv leads to such apparent loss of control over situs: 50% of iv/iv mice exhibit sit...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 86; no. 13; pp. 5035 - 5038
Main Authors Brueckner, Martina, D'Eustachio, Peter, Horwich, Arthur L.
Format Journal Article
LanguageEnglish
Published Washington, DC National Academy of Sciences of the United States of America 01.07.1989
National Acad Sciences
Subjects
Alleles
Animals
Biological and medical sciences
Chromosome Mapping
Chromosomes
Crosses, Genetic
DNA
DNA probes
Female
Fundamental and applied biological sciences. Psychology
Genes
Genes. Genome
Genetic Linkage
Genetic loci
Genetic mutation
Heart - anatomy & histology
Male
Medical genetics
Mice
Mice, Inbred Strains - anatomy & histology
Mice, Inbred Strains - genetics
Molecular and cellular biology
Molecular genetics
Mutation
Recombination, Genetic
Situs inversus
Species Specificity
Viscera - anatomy & histology
Heart
Vertebrata
Mammalia
Restriction fragment length polymorphism
Mouse
Molecular structure
Rodentia
Development
Organ
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Summary:Inherited single gene defects have been identified in both humans and mice that lead to loss of developmental control over the left-right asymmetry of the heart and viscera. In mice the recessively inherited mutation iv leads to such apparent loss of control over situs: 50% of iv/iv mice exhibit situs inversus and 50% exhibit normal situs. The affected gene product has not been identified in these animals. To study the normal function of iv, we have taken an approach directed to the gene itself. As a first step, we have mapped iv genetically, by examining its segregation in backcrosses with respect to markers defined by restriction fragment length polymorphisms. The iv locus lies 3 centimorgans (cM) from the immunoglobulin heavy-chain constant-region gene complex (Igh-C) on chromosome 12. A multilocus map of the region suggests the gene order centromere-Aat (α1-antitrypsin gene complex)-(11 cM)-iv-(3 cM)-Igh-C-(1 cM)-Igh-V (immunoglobulin heavy-chain variable-region gene complex).
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.13.5035