Seed germination of medicinal plant, fennel (Foeniculum vulgare Mill), as affected by different priming techniques

• Published in: Applied biochemistry and biotechnology Vol. 180; no. 1; pp. 26 - 40
• Main Authors: Tahaei, Amirreza, Soleymani, Ali, Shams, Majid
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.09.2016; Springer Nature B.V
• Subjects: Analysis of Variance; Biochemistry; Biotechnology; Chemistry; Chemistry and Materials Science; Distilled water; Foeniculum - drug effects; Foeniculum - genetics; Foeniculum - growth & development; Foeniculum vulgare; Genotype; Genotypes; Germination; Germination - drug effects; Growth chambers; Herbs; Medicinal plants; Plant Dormancy - drug effects; Plant growth; Plants, Medicinal - drug effects; Plants, Medicinal - growth & development; Polyethylene Glycols - pharmacology; Seed germination; Seedlings; Seedlings - drug effects; Seedlings - growth & development; Seeds; Seeds - drug effects; Seeds - growth & development; Fennel; Mill; Priming techniques; Seedling growth; Seed germination; Fennel (Foeniculum vulgare Mill)
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-016-2082-z

Reduced seed germination is among the most important factors adversely affecting crop stand and subsequent plant growth. Fennel ( Foeniculum vulgare Mill) is an important medicinal plant with poor seed germination rate, occasionally. It is accordingly pertinent to find methods which can enhance fennel seed germination and remove the barriers of dormancy breaking. The present experiments studied the effects of two different priming (cold moist stratification and osmopriming) and 14 dormancy breaking techniques (hormonal, osmopriming, biopriming, chemical priming, and hydropriming) on the seed germination and seedling growth of two different fennel genotypes under growth chamber conditions. In the first and second experiment, the priming techniques including the time lengths of cold moist stratification (0, 15, 30, and 45 days) and the concentrations of polyethylene glycol 6000 (PEG 6000 , osmopriming at −0.99, −1.35, and −2.33 MPa) were used as the main plots. However, in both experiments, the dormancy breaking techniques and fennel genotypes were factorially combined and used as the subplots. Different seed- and seedling-related parameters including germination (%), plumule, radicle and seedling length, average germination time, rate and homogeneity of germination, and seed vigor index were determined. Both priming techniques were efficient on the enhancement of seed germination and seedling growth. Among the dormancy breaking techniques, Aminol Forte (biopriming), kadostim (biopriming), benzyl adenine + kinetin (biopriming), distilled water (hydropriming), gibberellin + kinetin (hormonal priming), and benzyl adenine + kinetin + gibberellin (biopriming) were the most effective ones. The related concentrations were equal to 100 mg/l, 10 −5  M, and 0.4 %. The fennel genotypes reacted significantly different under priming conditions. It is possible to enhance seed germination and seedling growth of fennel using priming and dormancy breaking techniques, which is useful for the increased production of fennel under different conditions. The results indicate that bio and hydropriming techniques were among the most effective ones, which significantly increased seed germination and seedling growth, and removed the seed dormancy barriers.