Phytochemical Prospection and Modulation of Antibiotic Activity In Vitro by Lippia origanoides H.B.K. in Methicillin Resistant Staphylococcus aureus

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 7
• Main Authors: Cerqueira Fontinele, Filipe, Medeiros Barreto, Humberto, Pereira de Oliveira, Aldeídia, Arcanjo, Daniel Dias Rufino, Cavalcanti dos Santos, Bernadete Helena, de Abreu, Aislan Pereira Lira, Douglas Melo Coutinho, Henrique, Alves Carvalho da Silva, Romezio, Oliveira de Sousa, Taciana, Freire de Medeiros, Maria das Graças, Lopes Citó, Antonia Maria das Graças, Dantas Lopes, José Arimateia
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Acetates - chemistry; Anti-Bacterial Agents - pharmacology; Antibiotics; Antimicrobial agents; Bacteria; Care and treatment; Chlorpromazine - pharmacology; Ethanol; Ethanol - chemistry; Flavanones - pharmacology; Gas Chromatography-Mass Spectrometry; Health aspects; Herbal medicine; Hexanes - chemistry; Lippia; Lippia - chemistry; Medicinal plants; Metabolites; Methicillin-Resistant Staphylococcus aureus - drug effects; Methylene Chloride - chemistry; Microbial Sensitivity Tests; Monoterpenes - pharmacology; Pharmacology, Experimental; Phytochemicals; Phytochemicals - pharmacology; Plant Extracts - pharmacology; Staphylococcus aureus; Staphylococcus aureus infections; Staphylococcus infections; Thymol - pharmacology; Verbena
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/305610

The Lippia origanoides H.B.K. ethanol extract (LOEE) and hexane (LOHEX), dichloromethane (LODCM), and ethyl acetate (LOEA) fractions were tested for their antimicrobial activity alone or in combination with antibiotics against a methicillin resistant Staphylococcus aureus (MRSA) strain. The natural products did not show antimicrobial activity against multidrug resistant strain at the clinically significant concentrations tested. However, a modulatory effect in the antibacterial activity of the neomycin and amikacin was verified when LOEE, LOHEX and LODCM were added to the growth medium at subinhibitory concentrations. A similar modulation was found when the natural products were changed for chlorpromazine, an inhibitor of bacterial efflux pumps, suggesting the involvement of resistance mediated by efflux system in the MRSA tested. The fractions LOHEX and LODCM showed a modulatory activity bigger than their majority compounds (carvacrol, thymol, and naringenin), indicating that this activity is not due to their majority compounds only, but it is probably due to a synergism between their chemical components. These results indicate that L. origanoides H.B.K. can be a source of phytochemicals able to modify the phenotype of resistance to aminoglycosides in MRSA.