Flagellimonas eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome

• Published in: International journal of systematic and evolutionary microbiology Vol. 57; no. 5; pp. 1050 - 1054
• Main Authors: Bae, Seung Seob, Kwon, Kae Kyoung, Yang, Sung Hyun, Lee, Hee-Soon, Kim, Sang-Jin, Lee, Jung-Hyun
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.05.2007; Society for General Microbiology
• Subjects: Bacteria; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Calcium - metabolism; Carotenoids - analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Ecklonia kurome; Fatty Acids - analysis; Flagellimonas eckloniae; Flavobacteriaceae; Flavobacteriaceae - chemistry; Flavobacteriaceae - classification; Flavobacteriaceae - isolation & purification; Flavobacteriaceae - physiology; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Hydrogen-Ion Concentration; Korea; Marine; Microbiology; Molecular Sequence Data; Phaeophyceae - microbiology; Phylogeny; Pigments, Biological - biosynthesis; Quinones - analysis; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Sodium Chloride - metabolism; Systematics; Temperature; Asia; Korea; INW, Korea, Rep; Marine environment; Rhizosphere; New genus; Flavobacteriaceae; Taxonomy; Algae; Mesophily; Bacteria; Thallophyta
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.64565-0

Marine Biotechnology Center, Korea Ocean Research and Development Institute, Ansan PO Box 29, 425-600, Republic of Korea Correspondence Jung-Hyun Lee jlee{at}kordi.re.kr A marine bacterium, DOKDO 007 T , was isolated from the rhizosphere of the marine alga Ecklonia kurome collected from Dokdo Island, Korea, in October 2004. The strain produced orange-coloured colonies on marine agar 2216. 16S rRNA gene sequence analysis indicated that the novel isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarities with members of the genus Muricauda (92.0–94.0 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genera Muricauda and Costertonia . Cells were aerobic, Gram-negative rods producing non-diffusible carotenoid pigments. In contrast to all other members of the family Flavobacteriaceae , cells of DOKDO 007 T were motile by means of a polar flagellum. Optimal growth occurred in the presence of 3.5–4 % (w/v) sea salts (corresponding to 2.7–3.1 % NaCl), at pH 8 and at temperatures of 26–29 °C. The novel strain required Ca 2+ ions in addition to NaCl for growth. The dominant fatty acids were iso-15 : 0, iso-15 : 1 10 c and 10-methyl-16 : 0. The major respiratory quinone was MK-6. The DNA G+C content was 56.3 mol%, an unusually high value for members of the family Flavobacteriaceae . On the basis of these polyphasic taxonomic data, strain DOKDO 007 T should be classified as representing a new genus and novel species in the family Flavobacteriaceae , for which the name Flagellimonas eckloniae gen. nov., sp. nov. is proposed. The type strain is DOKDO 007 T (=KCCM 42307 T =JCM 13831 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DOKDO 007 T is DQ191180 . Transmission and scanning electron micrographs of cells of strain DOKDO 007 T are available as supplementary material in IJSEM Online.