Comprehensive analysis of a long non‑coding RNA‑associated competing endogenous RNA network in glioma

• Published in: Oncology letters Vol. 20; no. 4; p. 1
• Main Authors: Zhu, Jie, Wang, Han, Huang, Yue-Qing, Song, Wei, Li, Yi-Fan, Wang, Wen-Jie, Ding, Zhi-Liang
• Format: Journal Article
• Language: English
• Published: Athens Spandidos Publications 01.10.2020; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Analysis; Antigens; Brain cancer; Brain tumors; Cancer genetics; Cell cycle; Development and progression; Gene expression; Genes; Genetic aspects; Genomes; Genomics; Glioma; Gliomas; Hypotheses; Leukemia; Medical prognosis; Medical research; MicroRNA; MicroRNAs; Nervous system; Oncology; Perl; Proteins; RNA sequencing; Software; Survival analysis; Tumors; China
• ISSN: 1792-1074; 1792-1082
• DOI: 10.3892/ol.2020.11924

Long non-coding RNAs (IncRNAs) can act as competing endogenous RNAs (ceRNAs), interacting with microRNAs (miRNAs) and playing an important role in tumor progression. However, the role of IncRNA-mediated ceRNAs in glioma remains largely unknown. The present study aimed to identify novel IncRNAs and their associated function in glioma. RNA sequencing and corresponding clinical data from patients with glioma were obtained from The Cancer Genome Atlas. A total of 598 glioma tissues and 5 normal brain tissues were analyzed in the present study. The differentially expressed (DE) IncRNAs, mRNAs and miRNAs were identified using R packages and were used to construct a ceRNA network. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to investigate the biological functions of the DEmRNAs. Kaplan-Meier curve analysis was performed to investigate the association between DElncRNA expression and patient outcome. A total of 752 DElncRNAs, 2,079 DEmRNAs and 113 DEmiRNAs were identified between glioma and normal tissues. A IncRNA-miRNA-mRNA ceRNA network consisting of 61 IncRNAs, 12 miRNAs and 92 mRNAs was constructed. Survival analysis indicated that 36 DElncRNAs, 72 DEmRNAs and 3 DEmiRNAs were associated with overall survival in patients with glioma. The present study identified novel IncRNAs associated with survival prognosis and may facilitate further investigation of IncRNA-mediated ceRNA regulatory mechanisms in glioma.