A Defective Crosstalk Between Neurons and Müller Glial Cells in the rd1 Retina Impairs the Regenerative Potential of Glial Stem Cells
Müller glial cells (MGC) are stem cells in the retina. Although their regenerative capacity is very low in mammals, the use of MGC as stem cells to regenerate photoreceptors (PHRs) during retina degenerations, such as in retinitis pigmentosa, is being intensely studied. Changes affecting PHRs in dis...
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Published in | Frontiers in cellular neuroscience Vol. 13; p. 334 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Research Foundation
25.07.2019
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
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Summary: | Müller glial cells (MGC) are stem cells in the retina. Although their regenerative capacity is very low in mammals, the use of MGC as stem cells to regenerate photoreceptors (PHRs) during retina degenerations, such as in retinitis pigmentosa, is being intensely studied. Changes affecting PHRs in diseased retinas have been thoroughly investigated; however, whether MGC are also affected is still unclear. We here investigated whether MGC in
(
) mouse, an animal model of retinitis pigmentosa, have impaired stem cell properties or structure.
MGC showed an altered morphology, both in culture and in the whole retina. Using mixed neuron-glial cultures obtained from newborn mice retinas, we determined that proliferation was significantly lower in
than in
MGC. Levels of stem cell markers, such as
and
, were also markedly reduced in
MGC compared to
MGC in neuron-glial cultures and in retina cryosections, even before the onset of PHR degeneration. We then investigated whether neuron-glial crosstalk was involved in these changes. Noteworthy,
expression was restored in
MGC in co-culture with
neurons. Conversely,
expression decreased in
MGC in co-culture with
neurons, as occurred in
MGC in
neuron-glial mixed cultures. These results imply that MGC proliferation and stem cell markers are reduced in
retinas and might be restored by their interaction with "healthy" PHRs, suggesting that alterations in
PHRs lead to a disruption in neuron-glial crosstalk affecting the regenerative potential of MGC. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Mario Eduardo Guido, Center for Research in Biological Chemistry Córdoba (CIQUIBIC), Argentina Reviewed by: Antje Grosche, Ludwig-Maximilians-Universität München, Germany; Hugo Guerrero-Cazares, Mayo Clinic, United States These authors have contributed equally to this work This article was submitted to Cellular Neuropathology, a section of the journal Frontiers in Cellular Neuroscience |
ISSN: | 1662-5102 1662-5102 |
DOI: | 10.3389/fncel.2019.00334 |