Effect of dye localization and self-interactions on the photosensitized generation of singlet oxygen by rose bengal bound to bovine serum albumin

• Published in: Journal of photochemistry and photobiology. B, Biology Vol. 141; pp. 275 - 282
• Main Authors: Turbay, María Beatriz Espeche, Rey, Valentina, Argañaraz, Natalia M., Morán Vieyra, Faustino E., Aspée, Alexis, Lissi, Eduardo A., Borsarelli, Claudio D.
• Format: Journal Article
• Language: English
• Published: Switzerland Elsevier B.V 01.12.2014
• Subjects: Animals; Cattle; Hydrophobic and Hydrophilic Interactions; Kinetics; Photosensitizing Agents - chemistry; Photosensitizing Agents - metabolism; Protein Binding; Quantum Theory; Rose Bengal - chemistry; Rose Bengal - metabolism; Serum Albumin, Bovine - chemistry; Serum Albumin, Bovine - metabolism; Singlet Oxygen - chemistry; Singlet Oxygen - metabolism; Spectrometry, Fluorescence
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2014.09.014

[Display omitted] •Rose bengal (RB) binds efficiently to bovine serum albumin (BSA) at neutral pH.•Photophysical properties of RB depend on the average number of dye molecules per protein.•Multiple RB occupancy per BSA deactivates dye excited states.•One-to-one dye/protein adduct is an efficient supramolecular photosensitizer. The spectroscopic and photophysical properties of rose bengal (RB) encased in bovine serum albumin (BSA) have been examined to evaluate the photosensitized generation of singlet molecular oxygen (1O2). The results show that RB photophysical and photosensitizing properties are highly modulated by the average number of dye molecules per protein (n). At n≪1, the dye molecule is tightly located into the hydrophobic nanocavity site I of the BSA molecule with a binding constant Kb=0.15±0.01μM−1. The interaction with surrounding amino acids induces heterogeneous decay of both singlet and triplet excited states of RB and partially reduce its triplet quantum yield as compared with that in buffer solution. However, despite of the diffusive barrier imposed by the protein nanocavity to 3O2, the quenching of 3RB∗:BSA generates 1O2 with quantum yield ΦΔ=0.35±0.05. In turns, the intraprotein generated 1O2 is able to diffuse through the bulk solution, where is dynamically quenched by BSA itself with an overall quenching rate constant of 7.3×108M−1s−1. However, at n>1, nonspecific binding of up to ≈6RB molecules per BSA is produced, allowing efficient static quenching of excited states of RB preventing photosensitization of 1O2. These results provide useful information for development of dye-protein adducts suitable for using as potential intracellular photosensitizers.