Inflammation promotes airway epithelial ATP release via calcium-dependent vesicular pathways

• Published in: American journal of respiratory cell and molecular biology Vol. 49; no. 5; pp. 814 - 820
• Main Authors: Okada, Seiko F, Ribeiro, Carla M P, Sesma, Juliana I, Seminario-Vidal, Lucia, Abdullah, Lubna H, van Heusden, Catharina, Lazarowski, Eduardo R, Boucher, Richard C
• Format: Journal Article
• Language: English
• Published: United States American Thoracic Society 01.11.2013
• Subjects: Adenosine Triphosphate - metabolism; Calcium Signaling - drug effects; Cell Size; Cells, Cultured; Chelating Agents - pharmacology; Connexins - metabolism; Cystic Fibrosis - immunology; Cystic Fibrosis - metabolism; Epithelial Cells - drug effects; Epithelial Cells - immunology; Epithelial Cells - metabolism; Humans; Inflammation Mediators - metabolism; Interleukin-8 - metabolism; Mucins - metabolism; Mucociliary Clearance; Nerve Tissue Proteins - metabolism; Nucleotide Transport Proteins - metabolism; Osmotic Pressure; Pneumonia - immunology; Pneumonia - metabolism; Primary Cell Culture; Respiratory Mucosa - drug effects; Respiratory Mucosa - immunology; Respiratory Mucosa - metabolism; Secretory Vesicles - drug effects; Secretory Vesicles - immunology; Secretory Vesicles - metabolism; Time Factors
• ISSN: 1044-1549; 1535-4989
• DOI: 10.1165/rcmb.2012-0493oc

ATP in airway surface liquid (ASL) controls mucociliary clearance functions via the activation of airway epithelial purinergic receptors. However, abnormally elevated ATP levels have been reported in inflamed airways, suggesting that excessive ATP in ASL contributes to airway inflammation. Despite these observations, little is known about the mechanisms of ATP accumulation in the ASL covering inflamed airways. In this study, links between cystic fibrosis (CF)-associated airway inflammation and airway epithelial ATP release were investigated. Primary human bronchial epithelial (HBE) cells isolated from CF lungs exhibited enhanced IL-8 secretion after 6 to 11 days, but not 28 to 35 days, in culture, compared with normal HBE cells. Hypotonic cell swelling-promoted ATP release was increased in 6- to 11-day-old CF HBE cells compared with non-CF HBE cells, but returned to normal values after 28 to 35 days in culture. The exposure of non-CF HBE cells to airway secretions isolated from CF lungs, namely, sterile supernatants of mucopurulent material (SMM), also caused enhanced IL-8 secretion and increased ATP release. The SMM-induced increase in ATP release was sensitive to Ca(2+) chelation and vesicle trafficking/exocytosis inhibitors, but not to pannexin inhibition. Transcript levels of the vesicular nucleotide transporter, but not pannexin 1, were up-regulated after SMM exposure. SMM-treated cultures displayed increased basal mucin secretion, but mucin secretion was not enhanced in response to hypotonic challenge after the exposure of cells to either vehicle or SMM. We propose that CF airway inflammation up-regulates the capacity of airway epithelia to release ATP via Ca(2+)-dependent vesicular mechanisms not associated with mucin granule secretion.