Determination of Ethylglucuronide in Oral Fluid by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

• Published in: Journal of analytical toxicology Vol. 33; no. 4; pp. 204 - 207
• Main Authors: Hegstad, S., Johnsen, L., Mørland, J., Christophersen, A.S.
• Format: Journal Article
• Language: English
• Published: Niles, IL Oxford University Press 01.05.2009; Preston Publications
• Subjects: Alcohol Drinking - metabolism; Alcoholism and acute alcohol poisoning; Biological and medical sciences; Biotransformation; Calibration; Chromatography, Liquid - standards; Ethanol - metabolism; Glucuronates - metabolism; Humans; Medical sciences; Reproducibility of Results; Saliva - metabolism; Substance Abuse Detection - methods; Substance Abuse Detection - standards; Tandem Mass Spectrometry - standards; Time Factors; Toxicology; Validation; Ultra performance liquid chromatography; Solid phase extraction; Biological fluid; Tandem mass spectrometry; Chemical analysis; Ethanol; Metabolite; Coupled method; Alcohol; Electrospray; Glucuroconjugate; Chemical enrichment; Sample preparation; Saliva; Quantitative analysis
• ISSN: 0146-4760; 1945-2403
• DOI: 10.1093/jat/33.4.204

An ultra-performance liquid chromatography-tandem mass spectrometry method has been developed and validated for the determination of ethylglucuronide (EtG) in oral fluid. Sample clean-up was achieved by solid-phase extraction with a Hyper-SEP SAX column. Negative ionization was performed in the multiple reaction monitoring mode. Two transitions were monitored for the analyte and one for the internal standard EtG-d5. The calibration range was 4.4–222 ng/mL. The recovery of the analyte ranged from 86 to 99%, and the between-assay precisions ranged from 5 to 9% RSD. The limit of quantification was found to be 4.4 ng/mL. The concentration of EtG in oral fluid collected 2–14 h after a moderate alcohol intake varied from 13.3 to 57.7 ng/mL.