Association Between Programed Cell Death-1 and CD4 + T Cell Alterations in Different Phases of Ischemic Stroke Patients

• Published in: Frontiers in cellular neuroscience Vol. 12; p. 170
• Main Authors: Zhang, Yi, Wei, Li, Du, Yupeng, Xie, Yirui, Wu, Wei, Yuan, Yuan
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Research Foundation 22.06.2018; Frontiers Media S.A
• Subjects: Apoptosis; Bacterial infections; CD4 antigen; Cell death; Disease; Hospitals; Immunoglobulins; Immunology; Infections; Inflammation; Ischemia; ischemic stroke; Laboratories; Lymphocytes; Lymphocytes T; Medical research; Medicine; Mucin; Nervous system; Neuroscience; NMR; Nuclear magnetic resonance; PD-1; PD-1 protein; Peripheral blood; Phenotypes; Pneumonia; Roles; Stroke; stroke-induced immunodepression (SIID); T cells; Tim-3; Urogenital system; Viral infections; China; stroke-induced immunodepression (SIID); T cells; PD-1; ischemic stroke; Tim-3
• ISSN: 1662-5102; 1662-5102
• DOI: 10.3389/fncel.2018.00170

: We aimed to analyze alterations in T cell subgroups during different post-ischemic stroke (IS) phases to explore the possible mechanisms underlying stroke-induced immune depression (SIID). : Sixty-four IS patients who met the entry criteria were divided into three groups: an acute phase group, a sub-acute phase group and a stable phase group. Fourteen healthy individuals were selected as normal controls. The phenotype distribution of T cells in patient peripheral blood was analyzed, and the immune checkpoint receptors programed cell death-1 (PD-1) and T cell immunoglobulin and mucin domain 3 (Tim-3) were detected in different T cell phenotypes. : Compared with the control group, the absolute number of CD4 T cells and CD4 T central memory (TCM) cells was significantly increased in the acute phase group but decreased in the sub-acute phase and stable phase groups compared with that in the acute phase group. PD-1 expression in CD4 T cells in the stable phase group showed a significant increase compared with that in the acute phase group. The expression of PD-1 on CD4 TCM cells and CD4 T effector memory (TEM) cells showed significant decreases in the acute phase compared with control cells; however, in the sub-acute phase and the stable phase, PD-1 expression was significantly increased compared with that in the acute phase. : T cell dysfunction, especially CD4 T cell dysfunction, occurred during different IS phases. PD-1 was highly expressed in CD4 T cells of different phenotypes after the acute phase and was associated with alterations in CD4 T cells. Particularly, PD-1 was negatively correlated with the absolute number of TCM cells among different CD4 T cell phenotypes, which may be one of the possible mechanisms of SIID.