Enterococcus thailandicus sp. nov., isolated from fermented sausage ('mum') in Thailand

• Published in: International journal of systematic and evolutionary microbiology Vol. 58; no. 7; pp. 1630 - 1634
• Main Authors: Tanasupawat, Somboon, Sukontasing, Sirapan, Lee, Jung-Sook
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.07.2008; Society for General Microbiology
• Subjects: Bacteriology; Biological and medical sciences; Enterococcus; Enterococcus - classification; Enterococcus - genetics; Enterococcus - isolation & purification; Enterococcus durans; Enterococcus faecium; Enterococcus hirae; Fermentation; Food Microbiology; Fundamental and applied biological sciences. Psychology; Meat Products - microbiology; Microbiology; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - genetics; Species Specificity; Systematics; Thailand; Thailand; Asia; Enterococcus; Streptococcaceae; Lactic acid bacteria; Sausage; Taxonomy; Bacteria; Micrococcales; New species; Fermented product
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.65535-0

1 Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 2 Korean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea Correspondence Somboon Tanasupawat Somboon.T{at}chula.ac.th A Gram-positive and catalase-negative coccus that formed chains, designated strain FP48-3 T , isolated from fermented sausage (‘mum’), was studied systematically. Strain FP48-3 T was facultatively anaerobic and produced L -lactic acid from glucose. Straight-chain fatty acids C 18 : 1 and C 16 : 0 were the dominant components. The DNA G+C content of strain FP48-3 T was 37.9 mol%. On the basis of 16S rRNA and RNA polymerase -subunit ( rpoA ) gene sequence analysis, strain FP48-3 T was closely related to Enterococcus hirae LMG 6399 T , Enterococcus durans LMG 10746 T and Enterococcus faecium LMG 11423 T , with 99.3–99.6 and 95.1–96.9 % sequence similarities, respectively. Strain FP48-3 T could be clearly distinguished from E. hirae LMG 6399 T , E. durans LMG 10746 T and E. faecium LMG 11423 T by low DNA–DNA relatedness ( 14 %) and phenotypic characteristics. Therefore, this strain represents a novel species of the genus Enterococcus, for which the name Enterococcus thailandicus sp. nov. is proposed. The type strain is FP48-3 T (=KCTC 13134 T =NBRC 101867 T =NRIC 0107 T =TISTR 933 T =PCU 282 T ). The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and rpoA gene sequences of strain FP48-3 T are EF197994 and EU015878 , respectively. Neighbour-joining phylogenetic trees, based on 16S rRNA and rpoA gene sequences, showing the relationships between strain FP48-3 T and related species are available as supplementary material with the online version of this paper.