Biofilm Formation by Mycobacterium bovis : Influence of Surface Kind and Temperatures of Sanitizer Treatments on Biofilm Control

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 7
• Main Authors: Chen, Jinru, Bolatito, Olayemi K., Kehinde, Aderemi O., Adetunji, Victoria O.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Ammonium compounds; Ammonium paratungstate; Animals; Bacteriology; Benzylammonium Compounds - administration & dosage; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Cattle; Colony Count, Microbial; Health aspects; Iodine - administration & dosage; Microbial mats; Milk; Mycobacterium bovis; Mycobacterium bovis - growth & development; Mycobacterium bovis - pathogenicity; Stainless steel; Studies; Surface Properties; Temperature; Tuberculosis; Tuberculosis - drug therapy; Tuberculosis - microbiology; Tuberculosis - veterinary; Tuberculosis in cattle; United Kingdom; Nigeria
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/210165

Mycobacterium bovis causes classic bovine tuberculosis, a zoonosis which is still a concern in Africa. Biofilm forming ability of two Mycobacterium bovis strains was assessed on coupons of cement, ceramic, or stainless steel in three different microbiological media at 37°C with agitation for 2, 3, or 4 weeks to determine the medium that promotes biofilm. Biofilm mass accumulated on coupons was treated with 2 sanitizers (sanitizer A (5.5 mg L−1 active iodine) and sanitizer B (170.6 g1 alkyl dimethylbenzyl ammonium chloride, 78 g−1 didecyldimethyl ammonium chloride, 107.25 g L−1 glutaraldehyde, 146.25 g L−1 isopropanol, and 20 g L−1 pine oil) at 28 and 45°C and in hot water at 85°C for 5 min. Residual biofilms on treated coupons were quantified using crystal violet binding assay. The two strains had a similar ability to form biofilms on the three surfaces. More biofilms were developed in media containing 5% liver extract. Biofilm mass increased as incubation time increased till the 3rd week. More biofilms were formed on cement than on ceramic and stainless steel surfaces. Treatment with hot water at 85°C reduced biofilm mass, however, sanitizing treatments at 45°C removed more biofilms than at 28°C. However, neither treatment completely eliminated the biofilms. The choice of processing surface and temperatures used for sanitizing treatments had an impact on biofilm formation and its removal from solid surfaces.