Effect of coupling of albumin onto surface of PEG liposome on its in vivo disposition

• Published in: International journal of pharmaceutics Vol. 329; no. 1; pp. 110 - 116
• Main Authors: Furumoto, Kentaro, Yokoe, Jun-Ichi, Ogawara, Ken-ichi, Amano, Sayuri, Takaguchi, Maki, Higaki, Kazutaka, Kai, Toshiya, Kimura, Toshikiro
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.02.2007; Elsevier
• Subjects: Animals; Biological and medical sciences; Drug Delivery Systems; General pharmacology; Hepatic disposition; Liposomes; Male; Medical sciences; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Poly(ethylene glycol); Polyethylene Glycols; Rat serum albumin; Rats; Rats, Wistar; Serum Albumin - pharmacokinetics; Serum opsonins; Serum opsonins; Liposomes; Rat serum albumin; Hepatic disposition; Poly(ethylene glycol); Biological fluid; Pharmaceutical technology; Rat; Opsonin; Liver; Rodentia; Albumin; Liposome; Serum albumin; Ethylene oxide polymer; In vivo; Vertebrata; Mammalia; Animal; Serum; Pharmacokinetics
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2006.08.026

To evaluate the effect of coupling of albumin onto the surface of poly(ethylene glycol)-modified liposome (PEG liposome) on the in vivo disposition of liposome, pharmacokinetics and tissue distribution were examined after intravenous administration of rat serum albumin-modified PEG (RSA/PEG) liposome into rats. RSA/PEG liposome showed longer blood-circulating property than PEG liposome and the hepatic clearance for RSA/PEG liposome was significantly smaller than that for PEG liposome. Single-pass liver perfusion experiments also showed that the hepatic disposition of RSA/PEG liposome was much less than that of PEG liposome and that pre-treatment of liver with trypsin did not significantly reduce the hepatic disposition of RSA/PEG liposome, suggesting that RSA/PEG liposome could avoid the hepatic uptake via the receptor-mediated endocytosis. To unravel the mechanism behind the less affinity of RSA/PEG liposome to the liver, serum proteins associated on their surface were quantitatively and qualitatively assessed. The results showed that the coupling of albumin onto PEG liposome significantly reduced the total amount of serum proteins associated onto the surface, and SDS-PAGE revealed that the decrease in the association with liposomes for several serum proteins, which might have opsonic activity. From these findings, introduction of serum albumin onto PEG liposome could be useful to develop a new nanoparticulate formulation with a better pharmacokinetic property.