Narciclasine inhibits the responses of Arabidopsis roots to auxin

• Published in: Planta Vol. 236; no. 2; pp. 597 - 612
• Main Authors: Hu, Yanfeng, Yang, Lijing, Na, Xiaofan, You, Jia, Hu, Wei, Liang, Xiaolei, Liu, Jie, Mao, Lina, Wang, Xiaoming, Wang, Huahua, Bi, Yurong
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.08.2012; Springer; Springer Nature B.V
• Subjects: Agriculture; Amaryllidaceae Alkaloids - isolation & purification; Amaryllidaceae Alkaloids - pharmacology; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - physiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Auxins; Biodegradation; Biological and medical sciences; Biomedical and Life Sciences; Dose-Response Relationship, Drug; Ecology; Forestry; Fundamental and applied biological sciences. Psychology; Gene expression regulation; Gene Expression Regulation, Plant - drug effects; Genes; Genes, Reporter; Indoleacetic Acids - metabolism; Life Sciences; Original Article; Phenanthridines - isolation & purification; Phenanthridines - pharmacology; Phenotype; Plant cells; Plant growth; Plant Growth Regulators - metabolism; Plant roots; Plant Roots - drug effects; Plant Roots - genetics; Plant Roots - growth & development; Plant Roots - physiology; Plant Sciences; Plants; Promoter Regions, Genetic - genetics; Proteasome Endopeptidase Complex; Proteins; Proteolysis; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Root growth; Root hairs; Roots; Seedlings; Signal Transduction - drug effects; Signal Transduction - genetics; Auxin signaling; Aux/IAAs; SCF; Lateral root; ubiquitin protein ligase; Narciclasine; Proteasome; Multicatalytic endopeptidase complex; Auxin; Plant biology; Arabidopsis thaliana; Cruciferae; Ligases; Indoleacetic acid; Dicotyledones; Angiospermae; Plant growth substance; Ubiquitin-protein ligase; Carbon-nitrogen ligases; Root; Enzyme; Lateral; Response; Peptidases; Hydrolases; Spermatophyta; Experimental plant
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-012-1632-z

The plant hormone auxin plays a central role in the regulation of plant growth and development, as well as in responses to environmental stimuli. Narciclasine (NCS, an Amaryllidaceae alkaloid) isolated from Narcissus tazetta bulbs has a broad range of inhibitory effects on plants. In this study, the role of NCS in responses to auxin in Arabidopsis thaliana roots was investigated. We demonstrated the inhibitory effects of NCS on auxin-inducible lateral root formation, root hair formation, primary root growth, and the expression of primary auxin-inducible genes in Arabidopsis roots using DR5:: GUS reporter gene, native auxin promoters (IAA12::GUS, IAA13::GUS), and quantitative reverse transcription PCR analysis. Results also showed that NCS did not affect the expression of cytokinin-inducible ARR5::GUS reporter gene. NCS relieved the auxin-enhanced degradation of the Aux/IAA repressor modulated by the SCF TIR1 ubiquitin-proteasome pathway. In addition, NCS did not alter the auxin-stimulated interaction between IAA7/AXR2 (Aux/IAA proteins) and the F-box protein TIRI activity of the proteasome. Taken together, these results suggest that NCS acts on the auxin signaling pathway upstream of TIRI, which modulates Aux/IAA protein degradation, and thereby affects the auxin-mediated responses in Arabidopsis roots.