Effects of hyaluronic acid on peripheral nerve scarring and regeneration in rats

• Published in: Microsurgery Vol. 23; no. 6; pp. 575 - 581
• Main Author: Ozgenel, Güzin Yeşim
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 2003; Wiley-Liss
• Subjects: Administration, Topical; Animals; Biological and medical sciences; Biopsy, Needle; Cicatrix - drug therapy; Cicatrix - pathology; Disease Models, Animal; Hyaluronic Acid - pharmacology; Immunohistochemistry; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Nerve Regeneration - drug effects; Nerve Regeneration - physiology; Nervous system; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Peripheral Nerve Injuries; Probability; Rats; Rats, Sprague-Dawley; Sciatic Nerve; Sensitivity and Specificity; Statistics, Nonparametric; Peripheral nerve; Regeneration; Vertebrata; Experimental disease; Mammalia; Rat; Animal; Rodentia; Glycosaminoglycan; Hyaluronic acid; Biological activity
• ISSN: 0738-1085; 1098-2752
• DOI: 10.1002/micr.10209

The purpose of this experimental study was to investigate the effects of topical applications of hyaluronic acid on peripheral nerve scarring and regeneration in an adult rat model. After the right sciatic nerves of 48 rats were transected and immediately repaired, nerves were randomly divided into two groups. Nerves to which were applied hyaluronic acid comprised the experimental group, and nerves to which were applied saline comprised the control group. Perineural scarring was evaluated at 4 and 12 weeks macroscopically and histologically. Nerves treated with hyaluronic acid demonstrated significant reduction in perineural scar thickness (P < 0.05, Student's t‐test). Histomorphologic nerve analysis, electrophysiologic studies, muscle mass evaluation, and serial functional walking‐track analysis were performed for evaluation of peripheral nerve regeneration at 12 weeks. The results showed better conduction velocities, increased axon‐fiber diameter, and faster functional recovery in hyaluronic acid‐treated nerves (P < 0.05, Student's t‐test). In conclusion, hyaluronic acid appears to be effective in preventing perineural scar formation, resulting in enhancement of peripheral nerve regeneration. © 2003 Wiley‐Liss, Inc. MICROSURGERY 23:575–581 2003