The Role of MicroRNA, miR-24, and Its Target CHI3L1 in Osteomyelitis Caused by Staphylococcus aureus

• Published in: Journal of cellular biochemistry Vol. 116; no. 12; pp. 2804 - 2813
• Main Authors: Jin, Tao, Lu, Yong, He, Qing Xiao, Wang, Hai, Li, Bing Fu, Zhu, Liang Yue, Xu, Qing Yong
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.12.2015; Wiley Subscription Services, Inc
• Subjects: Adipokines - biosynthesis; Adipokines - genetics; Adipokines - metabolism; Adult; Apoptosis - genetics; Bone and Bones - metabolism; Bone and Bones - microbiology; Bone and Bones - pathology; Cell Differentiation - genetics; Cell Proliferation - genetics; CHI3L1; Chitinase-3-Like Protein 1; Female; Gene Expression Regulation; Humans; Lectins - biosynthesis; Lectins - genetics; Lectins - metabolism; Male; MicroRNAs - biosynthesis; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; miR-24; miRNA; Osteogenesis - genetics; OSTEOMYELITIS; Osteomyelitis - genetics; Osteomyelitis - microbiology; Osteomyelitis - pathology; Protein Binding; RNA, Messenger - biosynthesis; Signal Transduction - genetics; Staphylococcus aureus; Staphylococcus aureus - pathogenicity; CHI3L1; miRNA; OSTEOMYELITIS; miR-24; Staphylococcus aureus
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.25225

ABSTRACT Osteomyelitis is a debilitating infectious disease of the bone which is predominantly caused by Staphylococcus aureus (S. aureus). MicroRNAs (miRNAs) have been shown to play a regulatory role in osteogenesis. In the present study, the expression levels of miRNAs proposed to potentially play a regulatory role in bone formation or differentiation (miR‐24, miR‐29b, miR‐200a, miR‐208, miR‐322) were analyzed in the whole blood of patients with bacterial osteomyelitis or healthy controls, and in MC3T3‐E1 cells infected with S. aureus by qRT‐PCR. The expression of miR‐24 was significantly down‐regulated in osteomyelitis patients and S. aureus‐infected MC3T3‐E1 cells compared with the healthy controls or untreated control cells. Moreover, our results showed that S. aureus inhibited MC3T3‐E1 cell proliferation, induced osteoblast apoptosis and prohibited bone formation and mineralization. We found that overexpression of miR‐24 could reduce the effects of S. aureus, while inhibition of miR‐24 intensified the effects. We also demonstrated that miR‐24 suppressed the expression of chitinase 3‐like 1 (CHI3L1) mRNA, thought to mediate multiple signaling pathways, by directly binding to the 3′‐untranslated region. J. Cell. Biochem. 116: 2804–2813, 2015. © 2015 Wiley Periodicals, Inc.