Molecular Characterization of Two Silenced y-type Genes for Glu-B1 in Triticum aestivum ssp. yunnanese and ssp. tibetanum

• Published in: Journal of integrative plant biology Vol. 51; no. 1; pp. 93 - 99
• Main Authors: Yuan, Zhong-Wei, Chen, Qi-Jiao, Zhang, Lian-Quan, Yan, Ze-Hong, Zheng, You-Liang, Liu, Deng-Cai
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Melbourne, Australia : Blackwell Publishing Asia 2009; Blackwell Publishing Asia; Trlticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, China
• Subjects: 1By; Alleles; Amino Acid Sequence; Base Sequence; common wheat; Electrophoresis, Polyacrylamide Gel; gene silence; Gene Silencing; Genes, Plant; Glu-B1 locus; Glutens - analysis; Glutens - chemistry; Glutens - genetics; high molecular weight glutenin subunits; Molecular Sequence Data; Molecular Weight; Open Reading Frames - genetics; Polymerase Chain Reaction; Protein Subunits - analysis; Protein Subunits - chemistry; Protein Subunits - genetics; Sequence Homology, Amino Acid; Triticum; Triticum - genetics; Triticum aestivum; 1By; Glu-B1 locus; high molecular weight glutenin subunits; gene silence; common wheat
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/j.1744-7909.2008.00775.x

The high molecular weight glutenin subunits (HMW-GSs) are a major class of common wheat storage proteins. The bread-making quality of common wheat flour is influenced by the composition of HMW-GSs. In the present study, two unexpressed 1By genes from Triticum aesitvum L.ssp.yunnanese AS332 and T. aesitvum ssp.tibetanum AS908 were respectively cloned and characterized. The results indicated that both of the silenced 1By genes in AS332 and AS908 were 1By9. In contrast to previously reported mechanisms for silenced genes 1Ax and 1Ay, which was due to the insertion of transposon elements or the presence of premature stop codon via base substitution of C[rightward arrow]T transition in trinucleotides CAA or CAG, the silence of 1By9 genes was caused by premature stop codons via the deletion of base A in trinucleotide CAA, which lead to frameshift mutation and indirectly produced several premature stop codons (TAG) downstream of the coding sequence.