Human mast cells produce matrix metalloproteinase 9

Extracellular matrix‐destructive enzymes, like matrix metalloproteinases (MMP), have been recognized in the process of inflammation and tissue remodeling and repair. The affected tissues often contain markedly increased numbers of mast cells. Although mast cells are capable of activating latent coll...

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Published inEuropean journal of immunology Vol. 29; no. 8; pp. 2645 - 2649
Main Authors Kanbe, Naotomo, Tanaka, Akane, Kanbe, Michiyo, Itakura, Atsuko, Kurosawa, Motohiro, Matsuda, Hiroshi
Format Journal Article
LanguageEnglish
Published Weinheim WILEY‐VCH Verlag GmbH 01.08.1999
Subjects
Base Sequence
Cell Line
Collagenases - biosynthesis
Collagenases - genetics
DNA Primers - genetics
Fetal Blood - cytology
Fetal Blood - enzymology
Gene Expression
Humans
Immunohistochemistry
In Vitro Techniques
Infant, Newborn
Inflammation Mediators - metabolism
Mast cell
Mast Cells - enzymology
Matrix metalloproteinase
Matrix Metalloproteinase 9
phorbol 12-myristate 13-acetate
Remodeling
RNA, Messenger - genetics
RNA, Messenger - metabolism
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Summary:Extracellular matrix‐destructive enzymes, like matrix metalloproteinases (MMP), have been recognized in the process of inflammation and tissue remodeling and repair. The affected tissues often contain markedly increased numbers of mast cells. Although mast cells are capable of activating latent collagenase and proMMP, it has so far been unknown whether human mast cells themselves produce and secrete MMP9. In this study, MMP9 production by cord blood‐derived cultured human mast cells and HMC‐1 human mast cells was examined by reverse‐transcriptase PCR, gelatin zymography and Western blot analysis using an antibody against MMP9. Cultured mast cells and HMC‐1 cells treated with phorbol 12‐myristate 13‐acetate were shown to express MMP9 mRNA, and the cultured conditioned media from these cells showed gelatinolytic activity, identical with MMP9. Immunohistochemical examination was performed to detect MMP9 in tissue mast cells; mast cells localized in the skin, lung and synovial tissue showed strongly positive reactions for MMP9. Thus, these findings indicate that human mast cells can produce MMP9, which might contribute to extracellular matrix degradation and absorption in the process of allergic and nonallergic responses.
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ISSN:0014-2980
1521-4141
DOI:10.1002/(SICI)1521-4141(199908)29:08<2645::AID-IMMU2645>3.0.CO;2-1