Selective Acceleration of Arachidonic Acid Reincorporation into Brain Membrane Phospholipid Following Transient Ischemia in Awake Gerbil

• Published in: Journal of neurochemistry Vol. 70; no. 1; pp. 325 - 334
• Main Authors: Rabin, Olivier, Chang, Michael C. J., Grange, Eric, Bell, Jane, Rapoport, Stanley I., Deutsch, Joseph, Purdon, A. David
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.01.1998; Blackwell
• Subjects: Acyl Coenzyme A - metabolism; Acyl‐CoA; Animals; Arachidonic acid; Arachidonic Acid - metabolism; Autoradiography; Biological and medical sciences; Brain; Brain - metabolism; Fatty acid metabolism; Fatty Acids - metabolism; Fatty Acids, Nonesterified - metabolism; Gerbil; Gerbillinae; Image Processing, Computer-Assisted; Indicator Dilution Techniques; Ischemia; Ischemic Attack, Transient - metabolism; Male; Medical sciences; Membrane Lipids - metabolism; Neurology; Palmitates - metabolism; Palmitic acid; Phospholipid metabolism; Phospholipids - metabolism; Recovery; Reperfusion; Vascular diseases and vascular malformations of the nervous system; Nervous system diseases; Rodentia; Phospholipid; Cardiovascular disease; Metabolism; Arachidonic acid; Fatty acids; Cerebral disorder; Vascular disease; Vertebrata; Experimental disease; Mammalia; Ischemia; Animal; Central nervous system disease; Plasma membrane; Gerbil; Cerebrovascular disease; Brain (vertebrata)
• ISSN: 0022-3042; 1471-4159
• DOI: 10.1046/j.1471-4159.1998.70010325.x

: Awake gerbils were subjected to 5 min of forebrain ischemia by clamping the carotid arteries for 5 min and then allowing recirculation. Radiolabeled arachidonic or palmitic acid was infused intravenously for 5 min at the start of recirculation, after which the brains were prepared for quantitative autoradiography or chemical analysis. Dilution of specific activity of the acyl‐CoA pool was independently determined for these fatty acids in control gerbils and following 5 min of ischemia and 5 min of reperfusion. Using a quantitative method for measuring regional in vivo fatty acid incorporation into and turnover within brain phospholipids and determining unlabeled concentrations of acyl‐CoAs following recirculation, it was shown that reperfusion after 5 min of ischemia was accompanied by a threefold increase compared with the control in the rate of reincorporation of unlabeled arachidonate that had been released during ischemia, whereas reincorporation of released palmitate was not different from the control. Selective and accelerated reincorporation of arachidonate into brain phospholipids shortly after ischemia may ameliorate specific deleterious effects of arachidonate and its metabolites on brain membranes.