Application of monoclonal antibody, specific for intracellular Orientia tsutsugamushi, to immunofluorescent antibody test for determining antibiotic susceptibility

The simple quantification of viable intracellular bacteria is important for the study of an obligate intracellular bacterium, Orientia tsutsugamushi. We applied a novel monoclonal antibody (M686-13)--specific for intracellular Orientia--to an immunofluorescent antibody (IFA) test for determining ant...

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Published inMicrobiology and immunology Vol. 48; no. 9; p. 655
Main Authors Kim, Mee-Kyung, Odgerel, Zagga, Kim, Mi-Jeong, Chung, Moon-Hyun, Lim, Byung-Uk, Kang, Jae-Seung
Format Journal Article
LanguageEnglish
Published Australia 01.01.2004
Subjects
Anti-Bacterial Agents - pharmacology
Antibodies, Bacterial - immunology
Antibodies, Monoclonal - immunology
Antibody Specificity
Clindamycin - pharmacology
Doxycycline - pharmacology
Fluorescent Antibody Technique
Microbial Sensitivity Tests
Orientia tsutsugamushi - drug effects
Orientia tsutsugamushi - immunology
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Summary:The simple quantification of viable intracellular bacteria is important for the study of an obligate intracellular bacterium, Orientia tsutsugamushi. We applied a novel monoclonal antibody (M686-13)--specific for intracellular Orientia--to an immunofluorescent antibody (IFA) test for determining antibiotic susceptibility of O. tsutsugamushi. M686-13 did not react with Orientia that was inhibited by doxycycline, although bacterial particles still remained in the cells. This preferential staining of proliferating bacteria made the IFA test rapid and precise. Using this method, we could successfully measure the minimal inhibitory concentration (MIC) of a Korean strain of O. tsutsugamushi to doxycycline and clindamycin. This method may be used in other procedures to evaluate the growth of Orientia.
ISSN:0385-5600
DOI:10.1111/j.1348-0421.2004.tb03475.x