Detection via laser desorption and mass spectrometry of multiplex electrophore-labeled albumin

• Published in: Rapid communications in mass spectrometry Vol. 11; no. 16; pp. 1781 - 1784
• Main Authors: Bian, Nanying, Wang, Poguang, Wang, Zhixian, Xu, Linxiao, Church, George, Giese, Roger W.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.01.1997
• Subjects: Albumins - analysis; Gas Chromatography-Mass Spectrometry; Lasers; Mass Spectrometry; Solvents
• ISSN: 0951-4198; 1097-0231
• DOI: 10.1002/(SICI)1097-0231(19971030)11:16<1781::AID-RCM90>3.0.CO;2-F

Albumin was reacted with a mixture of six electrophore N‐hydroxysuccinimide esters, each of which possessed an interior glycolketo linkage. The purpose of this linkage is to release the attached electrophore as a ketone when heated, due to a thermal retro‐aldol reaction. The multiplex electrophore‐labeled albumin was detected as a dried spot deposited on a polyimide membrane by laser desorption/capillary collection (of the released ketone electrophores)/off‐line gas chromatography/electron caputre‐mass spectrometry. This encourages further study of such electrophore labels in immunoassays and related techniques, where there is a need to make advances in multi‐analyte detection. © 1997 John Wiley & Sons, Ltd.