Purification of coagulation factor VIII using chromatographic methods. Direct chromatography of plasma in anion exchange resins

• Published in: Biotechnology letters Vol. 32; no. 9; pp. 1207 - 1214
• Main Authors: Cheng, Elisabeth, Jinzenji, Daniela, Lorthiois, Ana Paula Almeida Aranha, de Carvalho, Roberta Rodrigues, Tanaka-Azevedo, Anita Mitiko, Raw, Isaías, Martins, Elizabeth Angélica Leme
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.09.2010; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Anion exchange; Anion Exchange Resins; Anion exchanging; Applied Microbiology; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Biotechnology - methods; Blood products; Chromatography; Chromatography, Gel; Chromatography, Ion Exchange - methods; Coagulation; Coagulation factors; Factor VIII - isolation & purification; Factor VIII/von Willebrand complexes; Filters; Fundamental and applied biological sciences. Psychology; Gel filtration; Hemophilia; Humans; Life Sciences; Microbiology; Original Research Paper; Plasma - chemistry; Plasma protein; Polymers; Purification; Resins; Technology, Pharmaceutical - methods; Vitamins; Coagulation factors; Factor VIII/von Willebrand complexes; Gel filtration; Anion exchange; Plasma protein; Purification; Factor VIII; Method; Complexes; Coagulation factor; Chromatography; Protein
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-010-0282-0

Coagulation factor VIII (FVIII) concentrates are used in the treatment of patients with Hemophilia A. Human FVIII was purified directly from plasma using anion exchange chromatography followed by gel filtration. Three Q-Sepharose resins were tested, resulting in 40% recovery of FVIII activity using Q-Sepharose XL resin, about 80% using Q-Sepharose Fast Flow and 70% using the Q-Sepharose Big Beads. The vitamin K-dependent coagulation factors co-eluted with FVIII from the anion exchange columns. In the second step of purification, when Sepharose 6FF was used, 70% of FVIII activity was recovered free from vitamin K-dependent factors.