Phytochemical constituents and hepatoprotective activity of Viburnum tinus

• Published in: Phytochemistry (Oxford) Vol. 66; no. 23; pp. 2780 - 2786
• Main Authors: Mohamed, Mona A., Marzouk, Mohamed S.A., Moharram, Fatma A., El-Sayed, Mortada M., Baiuomy, Ayman R.
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 01.12.2005; Elsevier
• Subjects: Acylated iridoid glucosides; Adoxaceae; Alanine Transaminase - drug effects; Alanine Transaminase - metabolism; animal physiology; Animals; Aspartate Aminotransferases - drug effects; Aspartate Aminotransferases - metabolism; Biochemistry & Molecular Biology; Biological and medical sciences; Carbon Tetrachloride - toxicity; Chemical constitution; chemical structure; coumarin; Coumarin sophoroside; Drug Evaluation, Preclinical; Fundamental and applied biological sciences. Psychology; General pharmacology; glucosides; Hepatoprotective activity; hepatoprotective effect; iridoid glycosides; Iridoids - chemistry; Iridoids - isolation & purification; Life Sciences & Biomedicine; Lipid peroxidation; Lipid Peroxides - blood; Liver - drug effects; Liver - physiology; Male; Medical sciences; medicinal plants; Molecular Structure; Nicotinic acid diethyl ester; Nitric oxide; Nitric Oxide - blood; Oleanolic Acid - chemistry; Oleanolic Acid - isolation & purification; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; phytochemicals; plant biochemistry; plant extracts; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant physiology and development; Plant Sciences; Plants, Medicinal - chemistry; Protective Agents - chemistry; Protective Agents - pharmacology; Rats; Saponins - chemistry; Saponins - isolation & purification; Science & Technology; scopoletin; sophoroside; spectral analysis; Viburnum - chemistry; Viburnum tinus; viburtinoside; Hepatoprotective activity; Acylated iridoid glucosides; Viburnum tinus; Coumarin sophoroside; Nitric oxide; Lipid peroxidation; Nicotinic acid diethyl ester; Adoxaceae; GLYCOSIDES; nitric oxide; IRIDOID GLUCOSIDES; acylated iridoid glucosides; nicotinic acid diethyl ester; hepatoprotective activity; lipid peroxidation; MEDICINAL-PLANTS; coumarin sophoroside; Iridoid; Caprifoliaceae; Rat; Molecular structure; Toxicity; Nicotinic acid; Lipids; Viburnum; Saponin; Ester; Dicotyledones; Angiospermae; Glycoside; Peroxides; Peroxidation; EC 2.6.1.2; EC 2.6.1.1; Rodentia; Glucoside; Plant leaf; Coumarin; Vertebrata; Mammalia; Flavone derivatives; Isolation; Spermatophyta
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2005.07.019

From the Leaves of Viburnum tinus L. four metabolites viz viburtinoside A and B (acylated iridoid glucosides), scopoletin 7- O-β- d-sophoroside and natural occurred 2,6-di- C-methyl-nicotinic acid 3,5-diethyl ester with 10 known metabolites were isolated. Toxicity and CCl 4-induced hepatotoxicity of the investigated extract have been evaluated in terms of the determination of ALT, AST, lipid peroxide and nitric oxide levels in serum. From the leaves of Viburnum tinus L. (Adoxaceae) two acylated iridoid glucosides (viburtinoside A and B), a coumarin diglucoside scopoletin 7- O-β- d-sophoroside and a natural occurred dinicotinic acid ester 2,6-di- C-methyl-nicotinic acid 3,5-diethyl ester were isolated. In addition to these, 10 known compounds were isolated, namely two bidesmosidic saponins, a hexamethoxy-flavone and five flavonol glycosides, as well as suspensolide A and oleanolic acid were isolated for the first time in this genus and species, respectively. The structures were determined mainly by spectroscopic methods (UV, IR, ESI-MS, 1H-, 13C NMR and DEPT). Toxicity of the investigated extract was determined (LD 50 = 500 mg/kg). CCl 4-induced hepatotoxicity has been evaluated in terms of the determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid peroxide and nitric oxide levels in serum and compared using adult male rats weighing 150–180 g. Their highly elevated levels were significantly reduced by treatment with the investigated aqueous methanol extract in dose-dependant manner.