An X chromosome-linked gene encoding a protein with characteristics of a rhoGAP predominantly expressed in hematopoietic cells

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 93; no. 2; pp. 695 - 699
• Main Authors: Tribioli, C, Droetto, S, Bione, S, Cesareni, G, Torrisi, M R, Lotti, L V, Lanfrancone, L, Toniolo, D, Pelicci, P
• Format: Journal Article
• Language: English
• Published: United States National Acad Sciences 23.01.1996; National Academy of Sciences
• Subjects: Actins - isolation & purification; Amino Acid Sequence; Base Sequence; Blotting, Northern; Cell Compartmentation; Cellular biology; DNA, Complementary - genetics; Gene Library; Genetic Linkage; Genetics; GTP-Binding Proteins - genetics; GTP-Binding Proteins - metabolism; GTPase-Activating Proteins; Hematopoietic Stem Cells - chemistry; Humans; Immunohistochemistry; Molecular Sequence Data; Proteins; Proteins - genetics; Proteins - metabolism; ras GTPase-Activating Proteins; ras Proteins - genetics; ras Proteins - isolation & purification; ras Proteins - metabolism; Sequence Analysis, DNA; Sequence Homology, Amino Acid; src Homology Domains; Tissue Distribution; X Chromosome - genetics
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.93.2.695

An increasingly large number of proteins involved in signal transduction have been identified in recent years and shown to control different steps of cell survival, proliferation, and differentiation. Among the genes recently identified at the tip of the long arm of the human X chromosome, a novel gene, C1, encodes a protein that appears to represent a newly discovered member of the group of signaling proteins involved in regulation of the small GTP binding proteins of the ras superfamily. The protein encoded by C1, p115, is synthesized predominantly in cells of hematopoietic origin. It is characterized by two regions of similarity to motifs present in known proteins: GAP and SH3 homologous regions. Its localization in a narrow cytoplasmic region just below the plasma membrane and its inhibitory effect on stress fiber organization indicate that p115 may down regulate rho-like GTPases in hematopoietic cells.