Synthetic biology toolkits and applications in Saccharomyces cerevisiae

Synthetic biologists construct biological components and systems to look into biological phenomena and drive a myriad of practical applications that aim to tackle current global challenges in energy, healthcare and the environment. While most tools have been established in bacteria, particularly Esc...

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Bibliographic Details
Published inBiotechnology advances Vol. 36; no. 7; pp. 1870 - 1881
Main Authors Chen, Binbin, Lee, Hui Ling, Heng, Yu Chyuan, Chua, Niying, Teo, Wei Suong, Choi, Won Jae, Leong, Susanna Su Jan, Foo, Jee Loon, Chang, Matthew Wook
Format Journal Article
LanguageEnglish
Published England Elsevier Inc 15.11.2018
Elsevier Science Ltd
Subjects
Abstraction
Applications
Baking yeast
Biology
Bioremediation
Chassis
Circuit design
E coli
Genetic toolkits
Genomes
Hierarchies
Saccharomyces cerevisiae
Synthetic biology
Toolkits
Yeast
Genetic toolkits
Synthetic biology
Yeast
Applications
Abstraction
Saccharomyces cerevisiae
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Summary:Synthetic biologists construct biological components and systems to look into biological phenomena and drive a myriad of practical applications that aim to tackle current global challenges in energy, healthcare and the environment. While most tools have been established in bacteria, particularly Escherichia coli, recent years have seen parallel developments in the model yeast strain Saccharomyces cerevisiae, one of the most well-understood eukaryotic biological system. Here, we outline the latest advances in yeast synthetic biology tools based on a framework of abstraction hierarchies of parts, circuits and genomes. In brief, the creation and characterization of biological parts are explored at the transcriptional, translational and post-translational levels. Using characterized parts as building block units, the designing of functional circuits is elaborated with examples. In addition, the status and potential applications of synthetic genomes as a genome level platform for biological system construction are also discussed. In addition to the development of a toolkit, we describe how those tools have been applied in the areas of drug production and screening, study of disease mechanisms, pollutant sensing and bioremediation. Finally, we provide a future outlook of yeast as a workhorse of eukaryotic genetics and a chosen chassis in this field.
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ISSN:0734-9750
1873-1899
DOI:10.1016/j.biotechadv.2018.07.005