Growth factor production from fibrin-encapsulated human keratinocytes

• Published in: Biotechnology letters Vol. 32; no. 7; pp. 1011 - 1017
• Main Authors: Acevedo, Cristian A, Somoza, Rodrigo A, Weinstein-Oppenheimer, Caroline, Brown, Donald I, Young, Manuel E
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.07.2010; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Applied Microbiology; Biochemistry; Biological and medical sciences; Biological properties; Biology; Biomedical and Life Sciences; Biotechnology; Cell Culture Techniques; Cells; Cells, Cultured; Culture; Encapsulation; Fibrin; Fibrin - metabolism; Fundamental and applied biological sciences. Psychology; Growth factors; Human; Humans; Intercellular Signaling Peptides and Proteins - secretion; keratinocytes; Keratinocytes - metabolism; Life Sciences; Microbiology; Monolayers; Original Research Paper; Proteins; Secretions; Tissue engineering; Keratinocytes; Fibrin; Growth factors; Encapsulation; Human; Production; Keratinocyte; Growth factor
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-010-0245-5

Fibrin has been used extensively in cell encapsulation because it has important biological properties. Keratinocyte encapsulation in fibrin is a widely used technique in skin tissue engineering. The production of growth factors (EGF, TGF-β1 and PDGF-BB) was evaluated when keratinocytes are encapsulated in fibrin. Secretions of TGF-β1 and PDGF-BB increased more than five times compared to monolayer cultures. Encapsulated cells secreted about 80% active form of TGF-β1 (monolayer cells only secreted inactive form). An enhanced secretion of TGF-β1 and PDGF-BB was found in encapsulated cells, showing that fibrin capsules are favourable for the production of these growth factors.