Metabolism of Scenedesmus obliquus cultivated with raw plant substrates
The potential benefits of adding raw, non-food, lignocellulosic plant material as a carbon source for mixotrophic growth of microalgae have previously been demonstrated. This approach has advantages over using traditional carbon sources like glucose or acetate due to wide-spread plant biomass availa...
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Published in | Frontiers in plant science Vol. 13; p. 992702 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media SA
02.12.2022
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
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Summary: | The potential benefits of adding raw, non-food, lignocellulosic plant material as a carbon source for mixotrophic growth of microalgae have previously been demonstrated. This approach has advantages over using traditional carbon sources like glucose or acetate due to wide-spread plant biomass availability and substrate recalcitrance to bacterial contamination. Here, we report the overall growth characteristics and explore the metabolic patterns of
cultured in the presence raw plant substrate. An initial screen of plant substrate candidates showed an increase in specific growth rate and biomass accumulation when
was cultured in the presence of switchgrass or yard waste compared to media alone. We observed a near doubling of microalgal dry weight when
was grown with 0.2% (
) switchgrass under ambient CO
. Scanning electron microscopy (SEM) of corn stem after
cultivation exhibited substantial phloem degradation. Transcriptomic analyses of
during mid- and late-log phase growth revealed a dynamic metabolic landscape within many KEGG pathways. Notably, differential expression was observed for several potential glycosyl hydrolases. We also investigated the influence of switchgrass on the growth of
at 50 L volume in mini raceway ponds to determine the scalability of this approach. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 USDOE This article was submitted to Marine and Freshwater Plants, a section of the journal Frontiers in Plant Science Edited by: Ben Lucker, Prosel Biosciences, United States Reviewed by: Bo Wang, Vanderbilt University, United States; Joshua Adam Temple, Michigan State University, United States |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2022.992702 |