Directed evolution of endoglucanase III (Cel12A) from Trichoderma reesei

• Published in: Applied microbiology and biotechnology Vol. 83; no. 4; pp. 649 - 657
• Main Authors: Nakazawa, Hikaru, Okada, Katsunori, Onodera, Tomoko, Ogasawara, Wataru, Okada, Hirofumi, Morikawa, Yasushi
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.06.2009; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: Amino acids; Animals; Biological and medical sciences; Biomass energy; Biotechnologically Relevant Enzymes and Proteins; Biotechnology; Carboxymethylcellulose Sodium - metabolism; Cats; Cellulase - chemistry; Cellulase - genetics; Cellulase - metabolism; Cellulose; Cloning; Directed Molecular Evolution; DNA polymerase; DNA, Fungal - genetics; E coli; Enzyme Stability; Enzymes; Escherichia coli - genetics; Evolution; Fundamental and applied biological sciences. Psychology; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Genetic engineering; Hydrogen-Ion Concentration; Kinetics; Life Sciences; Methods. Procedures. Technologies; Microbial Genetics and Genomics; Microbiology; Mutagenesis; Mutants; Plasmids; Polymerase Chain Reaction - methods; Protein engineering; Studies; Substrate Specificity; Temperature; Thermal cycling; Trichoderma - enzymology; Endoglucanase; Directed evolution; Error-prone PCR; Fungi; Directed molecular evolution; Polymerase chain reaction; Fungi Imperfecti; Trichoderma reesei
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-009-1901-3

The stability and specific activity of endo-β-1,4-glucanase III from Trichoderma reesei QM9414 was enhanced, and the expression efficiency of its encoding gene, egl3, was optimized by directed evolution using error-prone PCR and activity screening in Escherichia coli RosettaBlue (DE3) pLacI as a host. Relationship between increase in yield of active enzyme in the clones and improvement in its stability was observed among the mutants obtained in the present study. The clone harboring the best mutant 2R4 (G41E/T110P/K173M/Y195F/P201S/N218I) selected in via second-round mutagenesis after optimal recombinating of first-round mutations produced 130-fold higher amount of mutant enzyme than the transformant with wild-type EG III. Mutant 2R4 produced by the clone showed broad pH stability (4.4-8.8) and thermotolerance (entirely active at 55°C for 30 min) compared with those of the wild-type EG III (pH stability, 4.4-5.2; thermostability, inactive at 55°C for 30 min). k cat of 2R4 against carboxymethyl-cellulose was about 1.4-fold higher than that of the wild type, though the K m became twice of that of the wild type.