Towards a reliable technology for antioxidant capacity and oxidative damage evaluation: Electrochemical (bio)sensors

• Published in: Biosensors & bioelectronics Vol. 30; no. 1; pp. 1 - 12
• Main Authors: Barroso, M. Fátima, de-los-Santos-Álvarez, N., Delerue-Matos, C., Oliveira, M.B.P.P.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.12.2011; Elsevier
• Subjects: Antioxidants; Antioxidants - analysis; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensors; Biotechnology; Chemically modified electrode; Computer-Aided Design; Conductometry - instrumentation; DNA-based biosensor; Electrochemistry; Enzymatic biosensors; Equipment Design; Equipment Failure Analysis; Food Analysis - instrumentation; Free radicals; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Oligonucleotide Array Sequence Analysis - instrumentation; Oxidative Stress; Various methods and equipments; Antioxidants; Electrochemistry; Free radicals; Enzymatic biosensors; DNA-based biosensor; Chemically modified electrode; Antioxidant; Free radical; Electrodes; Chemical modification; Detector; Technology; Biosensor; DNA; Lesion
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2011.08.036

To counteract and prevent the deleterious effect of free radicals the living organisms have developed complex endogenous and exogenous antioxidant systems. Several analytical methodologies have been proposed in order to quantify antioxidants in food, beverages and biological fluids. This paper revises the electroanalytical approaches developed for the assessment of the total or individual antioxidant capacity. Four electrochemical sensing approaches have been identified, based on the direct electrochemical detection of antioxidant at bare or chemically modified electrodes, and using enzymatic and DNA-based biosensors.