Effects of Bauhinia forficata Tea on Oxidative Stress and Liver Damage in Diabetic Mice

• Published in: Oxidative medicine and cellular longevity Vol. 2016; no. 2016; pp. 1 - 9
• Main Authors: Puntel, Gustavo Orione, Puntel, Robson Luiz, Posser, Thaís, Zemolin, A. P. P., Mendez, Andreas S. L., da Silva, Marianne Pires, Folmer, Vanderlei, Salgueiro, Andréia Caroline Fernandes, Franco, Jeferson Luis
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2016; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Analysis; Animals; Antioxidants; Bauhinia - chemistry; Biomedical research; Blood Glucose - metabolism; Blotting, Western; Body Weight - drug effects; Catalase - metabolism; Chromatography, High Pressure Liquid; Dextrose; Diabetes; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - enzymology; Diabetes Mellitus, Experimental - pathology; Drinking water; Enzymes; Glucose; Heat shock proteins; HSP70 Heat-Shock Proteins; Hyperglycemia; Investigations; Lipid peroxidation; Liver; Liver - drug effects; Liver - pathology; Liver diseases; Mice; NAD(P)H Dehydrogenase (Quinone) - metabolism; NF-E2-Related Factor 2 - metabolism; Organ Size - drug effects; Oxidative stress; Oxidative Stress - drug effects; Porphobilinogen Synthase - metabolism; Proteins; Rodents; Solvents; Superoxide; Superoxide Dismutase - metabolism; Teas, Herbal - toxicity; Thiobarbituric Acid Reactive Substances - metabolism; Brazil
• ISSN: 1942-0900; 1942-0994
• DOI: 10.1155/2016/8902954

This study was designed to evaluate the effects of Bauhinia forficata Link subsp. pruinosa (BF) tea on oxidative stress and liver damage in streptozotocin (STZ)-induced diabetic mice. Diabetic male mice have remained 30 days without any treatment. BF treatment started on day 31 and continued for 21 days as a drinking-water substitute. We evaluated (1) BF chemical composition; (2) glucose levels; (3) liver/body weight ratio and liver transaminases; (4) reactive oxygen species (ROS), lipid peroxidation, and protein carbonylation in liver; (5) superoxide dismutase (SOD) and catalase (CAT) activities in liver; (6) δ-aminolevulinate dehydratase (δ-ALA-D) and nonprotein thiols (NPSH) in liver; (7) Nrf2, NQO-1, and HSP70 levels in liver and pancreas. Phytochemical analyses identified four phenols compounds. Diabetic mice present high levels of NQO-1 in pancreas, increased levels of ROS and lipid peroxidation in liver, and decrease in CAT activity. BF treatment normalized all these parameters. BF did not normalize hyperglycemia, liver/body weight ratio, aspartate aminotransferase, protein carbonyl, NPSH levels, and δ-ALA-D activity. The raised oxidative stress seems to be a potential mechanism involved in liver damage in hyperglycemic conditions. Our results indicated that BF protective effect could be attributed to its antioxidant capacity, more than a hypoglycemic potential.